中华肝脏外科手术学电子杂志
中華肝髒外科手術學電子雜誌
중화간장외과수술학전자잡지
Chinese Journal of Hepatic Surgery
2015年
5期
314-317
,共4页
韩玉湘%欧珊珊%肖笑雨%姚景余%杨禄坤
韓玉湘%歐珊珊%肖笑雨%姚景餘%楊祿坤
한옥상%구산산%초소우%요경여%양록곤
肝%再灌注损伤%缺氧诱导因子1,α亚基%血管内皮生长因子类%大鼠
肝%再灌註損傷%缺氧誘導因子1,α亞基%血管內皮生長因子類%大鼠
간%재관주손상%결양유도인자1,α아기%혈관내피생장인자류%대서
Liver%Reperfusion injury%Hypoxia-inducible factor 1,alpha subunit%Vascular endothelial growth factors%Rats
目的：探讨缺血-再灌注损伤（IRI）大鼠肝组织缺氧诱导因子（HIF）-1α和VEGF的表达及其意义。方法按随机数字表法将16只SD大鼠分为IRI组和假手术组（Sham组），每组8只。IRI组用无损伤血管夹夹闭大鼠门静脉和肝动脉的分支，缺血45 min，再恢复灌注6 h；Sham组仅暴露肝门，不阻断肝血流。观察两组大鼠肝组织形态学的改变，检测血清ALT、AST水平，测定肝组织HIF-1α、VEGF的蛋白含量。两组数据比较采用t检验。结果光镜下可见IRI组大鼠肝细胞肿胀，空泡变性，炎症细胞浸润，肝窦扩张，肝细胞索排列紊乱，点状坏死；Sham组肝组织形态正常。IRI组血清ALT、AST分别为（1007±130）、（1307±72）U/L，较Sham组的（59±4）、（81±3）U/L明显升高（t=20.700，48.448；P<0.05）。IRI组肝组织HIF-1α、VEGF蛋白相对表达量分别为1.77±0.10、1.86±0.05，较Sham组的0.60±0.22、0.83±0.06明显增加（t=13.623，35.563；P<0.05）。结论 IRI早期大鼠肝组织HIF-1α和VEGF表达明显增加，可能起到减轻机体IRI的作用。
目的：探討缺血-再灌註損傷（IRI）大鼠肝組織缺氧誘導因子（HIF）-1α和VEGF的錶達及其意義。方法按隨機數字錶法將16隻SD大鼠分為IRI組和假手術組（Sham組），每組8隻。IRI組用無損傷血管夾夾閉大鼠門靜脈和肝動脈的分支，缺血45 min，再恢複灌註6 h；Sham組僅暴露肝門，不阻斷肝血流。觀察兩組大鼠肝組織形態學的改變，檢測血清ALT、AST水平，測定肝組織HIF-1α、VEGF的蛋白含量。兩組數據比較採用t檢驗。結果光鏡下可見IRI組大鼠肝細胞腫脹，空泡變性，炎癥細胞浸潤，肝竇擴張，肝細胞索排列紊亂，點狀壞死；Sham組肝組織形態正常。IRI組血清ALT、AST分彆為（1007±130）、（1307±72）U/L，較Sham組的（59±4）、（81±3）U/L明顯升高（t=20.700，48.448；P<0.05）。IRI組肝組織HIF-1α、VEGF蛋白相對錶達量分彆為1.77±0.10、1.86±0.05，較Sham組的0.60±0.22、0.83±0.06明顯增加（t=13.623，35.563；P<0.05）。結論 IRI早期大鼠肝組織HIF-1α和VEGF錶達明顯增加，可能起到減輕機體IRI的作用。
목적：탐토결혈-재관주손상（IRI）대서간조직결양유도인자（HIF）-1α화VEGF적표체급기의의。방법안수궤수자표법장16지SD대서분위IRI조화가수술조（Sham조），매조8지。IRI조용무손상혈관협협폐대서문정맥화간동맥적분지，결혈45 min，재회복관주6 h；Sham조부폭로간문，불조단간혈류。관찰량조대서간조직형태학적개변，검측혈청ALT、AST수평，측정간조직HIF-1α、VEGF적단백함량。량조수거비교채용t검험。결과광경하가견IRI조대서간세포종창，공포변성，염증세포침윤，간두확장，간세포색배렬문란，점상배사；Sham조간조직형태정상。IRI조혈청ALT、AST분별위（1007±130）、（1307±72）U/L，교Sham조적（59±4）、（81±3）U/L명현승고（t=20.700，48.448；P<0.05）。IRI조간조직HIF-1α、VEGF단백상대표체량분별위1.77±0.10、1.86±0.05，교Sham조적0.60±0.22、0.83±0.06명현증가（t=13.623，35.563；P<0.05）。결론 IRI조기대서간조직HIF-1α화VEGF표체명현증가，가능기도감경궤체IRI적작용。
ObjectiveTo investigate the expression and significance of hypoxia-inducible factor (HIF)-1α and vascular endothelial growth factor (VEGF) in liver tissues after ischemia-reperfusion injury (IRI) in rats.MethodsSixteen Sprague-Dawley (SD) rats were randomized into the IRI group and the Sham group according to the random number table, 8 rats in each group. The portal vein and branches of hepatic artery of the rats in the IRI group were clamped with the atraumatic vascular clamp. After 45 min of ischemia, reperfusion was performed for 6 h. The porta hepatis of rats in the Sham group was exposed and the hepatic blood flow was not occluded. The morphological changes of the liver tissues in two groups were observed. The serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were tested and the contents of HIF-1α and VEGF in liver tissues were determined. Data comparison between two groups was conducted usingt test.ResultsUnder light microscope, swelling, vacuolar degeneration of the liver cells, inflammatory cell infiltration, hepatic sinusoidal dilatation, disorganized hepatic cords and spotty necrosis were observed in the IRI group. The histomorphology of liver tissues in the Sham group was normal. The serum ALT and AST in the IRI group was respectively (1 007±130) and (1 307±72) U/L, which were significantly higher than (59±4) and (81±3) U/L in the Sham group (t=20.700, 48.448;P<0.05). The relative expression of HIF-1α and VEGF in liver tissues of IRI group was 1.77±0.10 and 1.86±0.05 respectively, which were significantly higher than 0.60±0.22 and 0.83±0.06 in the Sham group (t=13.623, 35.563;P<0.05).ConclusionThe expression of HIF-1αand VEGF in liver tissues increase significantly during early IRI, which may play a role in reducing the IRI of organism.
