CAJ | 학술논문

目的：探讨热休克蛋白27（HSP27）在调控人胃癌细胞系5-FU耐药中的作用机制。方法培养人胃癌细胞株HGC27、BGC823、SGC7901、MKN28，采用CCK法筛选5-FU敏感株和耐药株，采用流式细胞仪和western blot 检测4株胃癌细胞株中HSP27表达水平，转染HSP27-siRNA，观察siRNA干扰后5-FU敏感株和耐药株中HSP27蛋白表达，并将两细胞株分别与不同浓度5-Fu共培养，采用CCK法检测细胞的半数抑制浓度（ IC50）。结果 CCK法显示SGC7901细胞系是4株胃癌细胞中5-Fu相对耐药株，而HCG27是5-Fu相对敏感株。流式细胞仪显示SGC7901细胞株中HSP27表达水平最高为（72．10±1．89）％，而在HGC27细胞株中的表达水平最低为（22．12±1．33）％，各株表达水平比较差异有统计学意义（ P＜0．05）。转染HSP27-siRNA 后，各两株细胞HSP27蛋白表达水平均下降明显。与5-Fu共培养后， SGC7901细胞株转染组细胞半数抑制浓度降低，药物敏感性增强，与未转染组比较差异有统计学意义（ P＜0．05）。结论HSP27参与了SGC79015-FU耐药株中的细胞耐药，是诱导细胞多药耐药的重要分子机制，可以作为临床治疗靶点来提高胃癌患者对化疗的敏感性。
목적：탐토열휴극단백27（HSP27）재조공인위암세포계5-FU내약중적작용궤제。방법배양인위암세포주HGC27、BGC823、SGC7901、MKN28，채용CCK법사선5-FU민감주화내약주，채용류식세포의화western blot 검측4주위암세포주중HSP27표체수평，전염HSP27-siRNA，관찰siRNA간우후5-FU민감주화내약주중HSP27단백표체，병장량세포주분별여불동농도5-Fu공배양，채용CCK법검측세포적반수억제농도（ IC50）。결과 CCK법현시SGC7901세포계시4주위암세포중5-Fu상대내약주，이HCG27시5-Fu상대민감주。류식세포의현시SGC7901세포주중HSP27표체수평최고위（72．10±1．89）％，이재HGC27세포주중적표체수평최저위（22．12±1．33）％，각주표체수평비교차이유통계학의의（ P＜0．05）。전염HSP27-siRNA 후，각량주세포HSP27단백표체수평균하강명현。여5-Fu공배양후， SGC7901세포주전염조세포반수억제농도강저，약물민감성증강，여미전염조비교차이유통계학의의（ P＜0．05）。결론HSP27삼여료SGC79015-FU내약주중적세포내약，시유도세포다약내약적중요분자궤제，가이작위림상치료파점래제고위암환자대화료적민감성。
Objective To investigate the mechanism of HSP 27 of 5-FU resistant in human gastric cancer cell lines . Methods Human gastric cancer cell lines(HGC27,BGC823,SGC7901,MKN28)were cultured,and the cells which were resist-ant to 5-FU were selected by CCK assay and flow cytometry .The expression of HSP27 in these cells were detected by Western Blotting.And 2 cell lines were co-cultured with different concentration of 5-Fu,then the half inhibition concentration was detected by CCK assay.Results CCK assay showed that SGC7901 cell line was relatively resistant to 5-Fu,and the HCG27 cell line was relatively sensitive to 5-Fu.The flow cytometry results suggested that the expression of HSP 27 in the SGC7901 cells was the high-est(72.10 ±1.89)%.Nevertheless,it was the lowest in the HGC27 cells(22.12 ±1.33)%.After transfection by HSP27-siR-NA,the expression levels of HSP27 in two cell lines was significant decreased (P<0.05);however,co-cultured with 5-Fu,the half inhibition concentration of SGC 7901 cell lines was decreased ,meanwhile the sensitivity to 5-Fu was enhanced .Conclusion HSP27 is involved in the 5-Fu resistance of SGC7901 cells,it induced an important molecular mechanism of multi-drug resistance and could be applied as a therapeutic target in order to increase the sensitivity of chemotherapy .