CAJ | 학술논문

目的：检测胃癌组织中H．pylori感染及PAR2的表达情况，研究PAR2对H．pylori感染小鼠胃粘膜上皮细胞增殖的影响。方法应用Warthin-Starry银染法，检测57例胃癌组织与20例癌旁组织中H．pylori感染情况，免疫组织化学法检测PAR2的表达。培养40只SPF级C57BL／6小鼠，随机分为5组。1组为对照组，以灭菌PBS灌胃；其余4组为实验组，以H．pylori悉尼株灌胃。分别于末次灌胃后第7、7、14、21、28天将5组小鼠处死后，采用流式细胞法检测细胞周期。结果胃癌组织H．pylori感染阳性组PAR2表达水平明显高于H．pylori感染阴性组与对照组（P＜0．05），H．pylo-ri感染阴性组PAR2表达水平高于对照组（P＜0．05）。小鼠胃粘膜上皮细胞增殖指数（PI）第1组与第2组比较差异无统计学意义（P＞0．05），第3、4、5组与对照组比较差异有统计学意义（P＜0．05）。 PAR2表达量与PI成正相关（P＜0．05）。结论 H．pylori感染的胃癌组织中PAR2的表达更显著，PAR2可能诱导H．pylori感染小鼠胃粘膜上皮细胞增殖，与胃癌的发生、发展有关。
목적：검측위암조직중H．pylori감염급PAR2적표체정황，연구PAR2대H．pylori감염소서위점막상피세포증식적영향。방법응용Warthin-Starry은염법，검측57례위암조직여20례암방조직중H．pylori감염정황，면역조직화학법검측PAR2적표체。배양40지SPF급C57BL／6소서，수궤분위5조。1조위대조조，이멸균PBS관위；기여4조위실험조，이H．pylori실니주관위。분별우말차관위후제7、7、14、21、28천장5조소서처사후，채용류식세포법검측세포주기。결과위암조직H．pylori감염양성조PAR2표체수평명현고우H．pylori감염음성조여대조조（P＜0．05），H．pylo-ri감염음성조PAR2표체수평고우대조조（P＜0．05）。소서위점막상피세포증식지수（PI）제1조여제2조비교차이무통계학의의（P＞0．05），제3、4、5조여대조조비교차이유통계학의의（P＜0．05）。 PAR2표체량여PI성정상관（P＜0．05）。결론 H．pylori감염적위암조직중PAR2적표체경현저，PAR2가능유도H．pylori감염소서위점막상피세포증식，여위암적발생、발전유관。
Objective To explore the correlation between helicobacter pylori infection and the expression of proteinase activated receptor 2 (PAR2) in gastric cancer tissues,and to study the effect of PAR2 on the proliferation of gastric mucosa epi-thelial cells of the mice infected by helicobacter pylori .Methods 57 cases of gastric cancer tissues were collected to detect heli-cobacter pylori infection by Warthin-Starry silver staining method ,and 20 cases of paracancerous tissues were selected as the con-trols.The expressions of PAR2 were detected by immunohistochemistry for all cases .40 specific pathogen-free C57BL/6 mice were randomly divided into 5 groups .Group 1 was used as the control group and was taken sterile PBS by intragastric administration , while the other 4 groups were taken helicobacter pylori SS 1 suspension by intragastric administration .5 groups of mice were sacri-ficed after 7,7,14,21,28 days after the last intragastric administration .The expression of PAR2 was detected by immunohisto-chemistry.Proliferation index (PI)was calculated by flow cytometry .Results The expression rate of PAR2 in helicobacter pylori positive group were significantly higher than those in helicobacter pylori negative group (P<0.05),and the expression rate of PAR2 in gastric cancer tissues were significantly higher than that of control group (P<0.05).The difference between the PI of mice group 1 and 2 had no statistical significance (P>0.05),while the difference among the PI of mice group 3,4 and 5 had sta-tistical significance (P<0.05).The expression of PAR2 and PI were related positively(P<0.05).Conclusion The expression of PAR2 is higher in gastric cancer tissues infected by helicobacter pylori .PAR2 may induce the proliferation of gastric mucosa epithelial cells ,and may be associated with the development of gastric cancer .