CAJ | 학술논문

目的:研究琥珀酸对大鼠海马CA1区的离子型 Glu受体介导的兴奋性突触后电流的调节. 方法:采用红外可视脑片膜片钳技术,观察琥珀酸对大鼠海马CA1区的谷氨酸( Glu)能自发兴奋性突触后电流( sEPSCs)和微小兴奋性突触后电流( mEPSCs)的调节作用. 结果:琥珀酸能降低大鼠海马CA1区离子型Glu受体介导的sEPSCs和mEPSCs的电流幅度,而对其频率和半衰减时间均无影响. 1 μmol/L琥珀酸组sEPSCs和mEPSCs的电流幅度分别为(24.16±2.61) pA 和(23.36±2.73) pA,与对照组比较差异显著(P<0.01). 结论:琥珀酸对突触前Glu释放无影响,但可显著降低EPSCs的电流幅度,琥珀酸可作为一种神经调质影响突触后兴奋性活动.
목적:연구호박산대대서해마CA1구적리자형 Glu수체개도적흥강성돌촉후전류적조절. 방법:채용홍외가시뇌편막편겸기술,관찰호박산대대서해마CA1구적곡안산( Glu)능자발흥강성돌촉후전류( sEPSCs)화미소흥강성돌촉후전류( mEPSCs)적조절작용. 결과:호박산능강저대서해마CA1구리자형Glu수체개도적sEPSCs화mEPSCs적전류폭도,이대기빈솔화반쇠감시간균무영향. 1 μmol/L호박산조sEPSCs화mEPSCs적전류폭도분별위(24.16±2.61) pA 화(23.36±2.73) pA,여대조조비교차이현저(P<0.01). 결론:호박산대돌촉전Glu석방무영향,단가현저강저EPSCs적전류폭도,호박산가작위일충신경조질영향돌촉후흥강성활동.
Objective:To study regulation of succinic acid on ionotropic Glu-mediated miniature excitatory postsynaptic cur-rents ( mEPSCs) and spontaneous excitatory postsynaptic currents ( sEPSCs) in rat hippocampal CA1 neurons. Methods:The regula-tion of succinic acid on ionotropic Glu-mediated mEPSCs and sEPSCs in the rat hippocampal CA1 neurons were observed by using in-frared visual patch clamp technique. Results:Succinic acid could reduce the amplitudes of ionotropic Glu-mediated mEPSCs and sEP-SCs, but had no effect on the frequency and half-decay period. The amplitudes of mEPSCs and sEPSCs were (23. 36±2. 73) pA and (24. 16±2. 61) pA in the 1 μmol/L Succinic acid group, which had significant differences with control group (n=12, P<0. 01). Conclusions:Succinic acid has no effect on presynaptic Glu release, but can significantly reduce the current amplitude. Therefore, it may be a neuromodulator influencing postsynaptic excitatory activities.