天津科技大学学报
天津科技大學學報
천진과기대학학보
Journal of Tianjing University of Science & Technology
2015年
5期
32-36
,共5页
张锋%张洪锋%杨明非%顾皞
張鋒%張洪鋒%楊明非%顧皞
장봉%장홍봉%양명비%고호
水杨基荧光酮%荧光光谱%荧光量子产率%罗丹明6G
水楊基熒光酮%熒光光譜%熒光量子產率%囉丹明6G
수양기형광동%형광광보%형광양자산솔%라단명6G
Salicylfluorone%fluorescence spectra%fluorescence quantum yield%Rhodamine 6,G
研究了水杨基荧光酮(SAF)的荧光光谱和荧光量子产率，发现在pH 2.0以下，SAF无荧光，随pH升高，SAF由非荧光体转化为荧光体，荧光强度增强，在pH 6.0～7.0，SAF有稳定的强荧光，最大发射波长537,nm，最大激发波长507,nm．在碱性条件下，随pH升高，SAF荧光强度下降，当pH在10.0以上SAF无荧光．在pH＝6.5的缓冲溶液中， SAF 稀水溶液的荧光强度与浓度之间存在良好的线性关系，线性范围为2.0×10-7～1.2×10-6,mol/L，检出限为5.2×10-9,mol/L．SAF 在醇溶液中，激发波长蓝移，发射峰红移，Stokes 位移增大．在稀水溶液中，β-环糊精对 SAF 的荧光强度有很强的增敏作用．以罗丹明6,G 为参比，使用 Williams 梯度法测量了 SAF 的荧光量子产率，在激发波长500,nm处的荧光量子产率为0.68．
研究瞭水楊基熒光酮(SAF)的熒光光譜和熒光量子產率，髮現在pH 2.0以下，SAF無熒光，隨pH升高，SAF由非熒光體轉化為熒光體，熒光彊度增彊，在pH 6.0～7.0，SAF有穩定的彊熒光，最大髮射波長537,nm，最大激髮波長507,nm．在堿性條件下，隨pH升高，SAF熒光彊度下降，噹pH在10.0以上SAF無熒光．在pH＝6.5的緩遲溶液中， SAF 稀水溶液的熒光彊度與濃度之間存在良好的線性關繫，線性範圍為2.0×10-7～1.2×10-6,mol/L，檢齣限為5.2×10-9,mol/L．SAF 在醇溶液中，激髮波長藍移，髮射峰紅移，Stokes 位移增大．在稀水溶液中，β-環糊精對 SAF 的熒光彊度有很彊的增敏作用．以囉丹明6,G 為參比，使用 Williams 梯度法測量瞭 SAF 的熒光量子產率，在激髮波長500,nm處的熒光量子產率為0.68．
연구료수양기형광동(SAF)적형광광보화형광양자산솔，발현재pH 2.0이하，SAF무형광，수pH승고，SAF유비형광체전화위형광체，형광강도증강，재pH 6.0～7.0，SAF유은정적강형광，최대발사파장537,nm，최대격발파장507,nm．재감성조건하，수pH승고，SAF형광강도하강，당pH재10.0이상SAF무형광．재pH＝6.5적완충용액중， SAF 희수용액적형광강도여농도지간존재량호적선성관계，선성범위위2.0×10-7～1.2×10-6,mol/L，검출한위5.2×10-9,mol/L．SAF 재순용액중，격발파장람이，발사봉홍이，Stokes 위이증대．재희수용액중，β-배호정대 SAF 적형광강도유흔강적증민작용．이라단명6,G 위삼비，사용 Williams 제도법측량료 SAF 적형광양자산솔，재격발파장500,nm처적형광양자산솔위0.68．
Fluorescence spectra and fluorescence quantum yield of Salicylfluorone(SAF)have been studied. Under the con-dition of pH<2.0,SAF has no fluorescence. With the increase of pH value,SAF changes from non-luminous chemical to fluorophore and its fluorescence intensity increases. In the range of pH 6.0-7.0,SAF gives a strong and steady fluorescence with a maximum emission wavelength of 537,nm and excitation wavelength of 507,nm. In alkaline solution,fluorescence intensity of SAF decreases with the increase of pH value,and it has no fluorescence when pH>10.0. There is an excellent linear relationship between fluorescence intensity and the concentration of SAF in pH=6.5 buffer solution.The linear range is 2.0×10-7-1.2×10-6 mol/L,and the detection limit is 5.2×10-9 mol/L. In alcoholic solutions,Stokes drift of SAF in-creases as blue shift occurs on molecular excitation wavelength and red shift occurs on emission wavelength.β-cyclodextrin can be used as an excellent sensitizer because fluorescence intensity of SAF is enhanced enormously by addingβ-CD. Rho-damine 6,G was used as a reference,and fluorescence quantum yield of SAF was measured by means of Williams gradient method. At the excitation wavelength 500,nm,the fluorescence quantum yield of SAF is 0.68.
