CAJ | 학술논문

目的探讨15-脱氧-前列腺素J2(15-d-PGJ2)对肝癌细胞SMMC-7721侵袭转移的影响及其内在的机制.方法 SMMC-7721肝癌细胞经天然配体15-d-PGJ2和(或)抑制剂GW9662处理, 用伤口愈合实验和Transwell方法检测细胞的转移侵袭能力, 用RT-PCR检测细胞PTEN和MMP-9 mRNA的表达,用Western blotting检测细胞PTEN和MMP-9蛋白的表达. 结果 15-d-PGJ2对SMMC-7721细胞的转移侵袭具有抑制作用,并能上调PTEN mRNA和蛋白的表达,下调MMP-9 mRNA 和蛋白的表达; 单药 GW9662 无此效应, GW9662 与15-d-PGJ2 联合能够逆转此效应.结论 15-d-PGJ2是通过激活PPAR信号上调PTEN表达,下调MMP-9表达,抑制肝癌细胞的侵袭转移.
목적 탐토15-탈양-전렬선소J2(15-d-PGJ2)대간암세포SMMC-7721침습전이적영향급기내재적궤제.방법 SMMC-7721간암세포경천연배체15-d-PGJ2화(혹)억제제GW9662처리, 용상구유합실험화Transwell방법검측세포적전이침습능력, 용RT-PCR검측세포PTEN화MMP-9 mRNA적표체,용Western blotting검측세포PTEN화MMP-9단백적표체. 결과 15-d-PGJ2대SMMC-7721세포적전이침습구유억제작용,병능상조PTEN mRNA화단백적표체,하조MMP-9 mRNA 화단백적표체; 단약 GW9662 무차효응, GW9662 여15-d-PGJ2 연합능구역전차효응.결론 15-d-PGJ2시통과격활PPAR신호상조PTEN표체,하조MMP-9표체,억제간암세포적침습전이.
Objective To investigate the effect of 15-deoxy-prostaglandin J2 (15-d-PGJ2) on the invasion and metastasis of hepatocellular carcinoma SMMC-7721 cells, as well as its underlying mechanisms. Methods Hepatocellular carcinoma SMMC-7721 cells were treated by natural ligand 15-d-PGJ2 with or without antagonist GW9662 of peroxisome proliferator-activated receptor (PPAR). Invasion and metastasis of SMMC-7721 cells was measured by wound healing assay and Transwell chambers. mRNAs of PTEN and MMP-9 were assessed by RT-PCR and proteins by Western Blotting analysis, respectively. Results 15-d-PGJ2, but not GW9662, inhibited the invasion and metastasis of SMMC-7721 cells, up-regulated PTEN mRNA and protein expression, and down-regulated MMP-9 mRNA and protein expression. Howev-er, after SMMC-7721 cells were pretreated for 1 h with GW9662 and exposed to 15-d-PGJ2, the inhi-bitive effects of 15-d-PGJ2 on invasion and metastasis of cancer cells were reversed. Conclusion 15-d-PGJ2 increases PTEN expression and decreases MMP-9 expression to suppress the invasion and metastasis of HCC via activating PPAR signal pathway.