东南国防医药
東南國防醫藥
동남국방의약
Military Medical Journal of Southeast China
2015年
5期
458-460,495
,共4页
陈荣剑%祝仲珍%王占科%杨莉萍%袁小兰%胡新华%宁丽萍%兰小鹏
陳榮劍%祝仲珍%王佔科%楊莉萍%袁小蘭%鬍新華%寧麗萍%蘭小鵬
진영검%축중진%왕점과%양리평%원소란%호신화%저려평%란소붕
亚最低杀菌浓度%阿莫西林%体外诱导%细菌耐药%金黄色葡萄球菌
亞最低殺菌濃度%阿莫西林%體外誘導%細菌耐藥%金黃色葡萄毬菌
아최저살균농도%아막서림%체외유도%세균내약%금황색포도구균
sub-MBC%amoxicillin%induce in vitro%bacteria antibiotic%staphylococcus aureus
目的：观察亚最低杀菌浓度（ Sub－minimum bactericidal concentration，Sub－MBC）阿莫西林对金黄色葡萄球菌标准菌株的耐药性影响。方法以金黄色葡萄球菌标准菌株（ATCC 29213）为研究对象，阿莫西林为抗菌药物，以微量稀释法定量检测不同诱导天数MBC值，采用1／2MBC浓度对金黄色葡萄球菌进行30 d多步体外诱导实验，观察金黄色葡萄球菌标准菌株MBC变化，以全自动微生物药敏鉴定仪对最终诱导后菌株进行阿莫西林耐药性分析。结果金黄色葡萄球菌标准菌株MBC值阿莫西林体外诱导7 d即显著升高，诱导30 d升高至原始菌株MBC的32倍；经全自动药敏分析仪鉴定诱导出的耐药菌株对阿莫西林耐药。结论亚最低杀菌浓度阿莫西林可体外诱导金黄色葡萄球菌标准菌株出现耐药，且耐药性随亚最低杀菌浓度诱导时间增加而升高，提示合理选择抗菌药物使用剂量可作为预防细菌耐药重要措施。
目的：觀察亞最低殺菌濃度（ Sub－minimum bactericidal concentration，Sub－MBC）阿莫西林對金黃色葡萄毬菌標準菌株的耐藥性影響。方法以金黃色葡萄毬菌標準菌株（ATCC 29213）為研究對象，阿莫西林為抗菌藥物，以微量稀釋法定量檢測不同誘導天數MBC值，採用1／2MBC濃度對金黃色葡萄毬菌進行30 d多步體外誘導實驗，觀察金黃色葡萄毬菌標準菌株MBC變化，以全自動微生物藥敏鑒定儀對最終誘導後菌株進行阿莫西林耐藥性分析。結果金黃色葡萄毬菌標準菌株MBC值阿莫西林體外誘導7 d即顯著升高，誘導30 d升高至原始菌株MBC的32倍；經全自動藥敏分析儀鑒定誘導齣的耐藥菌株對阿莫西林耐藥。結論亞最低殺菌濃度阿莫西林可體外誘導金黃色葡萄毬菌標準菌株齣現耐藥，且耐藥性隨亞最低殺菌濃度誘導時間增加而升高，提示閤理選擇抗菌藥物使用劑量可作為預防細菌耐藥重要措施。
목적：관찰아최저살균농도（ Sub－minimum bactericidal concentration，Sub－MBC）아막서림대금황색포도구균표준균주적내약성영향。방법이금황색포도구균표준균주（ATCC 29213）위연구대상，아막서림위항균약물，이미량희석법정량검측불동유도천수MBC치，채용1／2MBC농도대금황색포도구균진행30 d다보체외유도실험，관찰금황색포도구균표준균주MBC변화，이전자동미생물약민감정의대최종유도후균주진행아막서림내약성분석。결과금황색포도구균표준균주MBC치아막서림체외유도7 d즉현저승고，유도30 d승고지원시균주MBC적32배；경전자동약민분석의감정유도출적내약균주대아막서림내약。결론아최저살균농도아막서림가체외유도금황색포도구균표준균주출현내약，차내약성수아최저살균농도유도시간증가이승고，제시합리선택항균약물사용제량가작위예방세균내약중요조시。
Objective To observe the effects of inducing by amoxicillin with the dose of sub-MBC( minimum bactericidal con-centration) on the antibiotic resistance of Standard strains of staphylococcus aureus.Methods The standard strain ATCC 29213 of staphylococcus aureus were induced by amoxicillin with the dose of 1/2MBC for 30 days.The quantitative data of MBC were detected by microdilution method in vitro everyday, and the antibiotic resistance to amoxicillin of staphylococcus aureus induced by this method were identified using automatic microorganisms drug sensitivity analyzer.Results The quantitative data of MBC of the standard strain of staphylococcus aureus induced by amoxicillin for 7 days significantly increased, and the MBC increased 32 times after induced for 30 day than that of the original strain.The drug-resistant strain induced by our methods showed resistant of amoxicillin which was identi-fied by the automatic microorganisms drug sensitivity analyzer.Conclusion Amoxicillin can induce the standard strains of staphylo-coccus aureus resistance to antibiotic with the dose of sub-MBC for long time in vitro, and the degree of the antibiotic resistance in-creased by the time of inducement with sub-MBC, suggesting that it is important to to select a rational dosage of antimicrobial to prevent antibiotic resistance in clinics.
