中华创伤杂志
中華創傷雜誌
중화창상잡지
Chinese Journal of Trauma
2015年
10期
949-953
,共5页
尹义国%孙兆良%陈二涛%冯东福
尹義國%孫兆良%陳二濤%馮東福
윤의국%손조량%진이도%풍동복
弥漫性轴索损伤%海马%吗替麦考酚酯
瀰漫性軸索損傷%海馬%嗎替麥攷酚酯
미만성축색손상%해마%마체맥고분지
Diffuse axonal injury%Hippocampus%Mycophenolate mofetil
目的 探讨吗替麦考酚酯(MMF)对弥漫性轴索损伤(DAI)大鼠海马区胶质瘢痕形成及学习记忆功能的影响. 方法 将96只成年SD大鼠按随机数字表法分为空白对照组、等渗盐水(NS)组和MMF组,每组32只.免疫组化染色检测DAI后大鼠海马区活化小胶质细胞、活化星形胶质细胞以及硫酸软骨素蛋白聚糖(CSPGs)的表达,Image-Pro Plus软件定量评估DAI易损区星形胶质细胞、小胶质细胞、CSPGs的表达量.Morris水迷宫试验检测各组大鼠伤后7～11d、28 ～ 32 d学习记忆功能.对各组伤后7,14,28 d胶质瘢痕主要成分累积吸光度(IA)值及伤后7～11d、28 ～ 32 d的Morris水迷宫参数进行统计分析,并对两者进行相关性分析. 结果 在各时相点,MMF组大鼠海马区胶质瘢痕主要成分IA值均低于NS组与空白对照组(P＜0.05);在伤后7～11d,各组大鼠Morris水迷宫参数(目标象限路程百分比和时间百分比)的差异无统计学意义且无任何相关性;在伤后28 ～ 32 d,MMF组大鼠Morris水迷宫参数(目标象限路程百分比和时间百分比)明显降低(P＜0.01);伤后28 d胶质瘢痕的主要成分IA值与DAI大鼠28 ～32 d Morris水迷宫参数的平均速度和逃匿潜伏期呈正相关关系,与目标象限停留路程百分比和停留时间百分比呈负相关关系(P＜0.01). 结论 早期应用MMF能够显著抑制DAI大鼠海马区胶质瘢痕形成,改善DAI大鼠恢复期的学习记忆功能.
目的 探討嗎替麥攷酚酯(MMF)對瀰漫性軸索損傷(DAI)大鼠海馬區膠質瘢痕形成及學習記憶功能的影響. 方法 將96隻成年SD大鼠按隨機數字錶法分為空白對照組、等滲鹽水(NS)組和MMF組,每組32隻.免疫組化染色檢測DAI後大鼠海馬區活化小膠質細胞、活化星形膠質細胞以及硫痠軟骨素蛋白聚糖(CSPGs)的錶達,Image-Pro Plus軟件定量評估DAI易損區星形膠質細胞、小膠質細胞、CSPGs的錶達量.Morris水迷宮試驗檢測各組大鼠傷後7～11d、28 ～ 32 d學習記憶功能.對各組傷後7,14,28 d膠質瘢痕主要成分纍積吸光度(IA)值及傷後7～11d、28 ～ 32 d的Morris水迷宮參數進行統計分析,併對兩者進行相關性分析. 結果 在各時相點,MMF組大鼠海馬區膠質瘢痕主要成分IA值均低于NS組與空白對照組(P＜0.05);在傷後7～11d,各組大鼠Morris水迷宮參數(目標象限路程百分比和時間百分比)的差異無統計學意義且無任何相關性;在傷後28 ～ 32 d,MMF組大鼠Morris水迷宮參數(目標象限路程百分比和時間百分比)明顯降低(P＜0.01);傷後28 d膠質瘢痕的主要成分IA值與DAI大鼠28 ～32 d Morris水迷宮參數的平均速度和逃匿潛伏期呈正相關關繫,與目標象限停留路程百分比和停留時間百分比呈負相關關繫(P＜0.01). 結論 早期應用MMF能夠顯著抑製DAI大鼠海馬區膠質瘢痕形成,改善DAI大鼠恢複期的學習記憶功能.
목적 탐토마체맥고분지(MMF)대미만성축색손상(DAI)대서해마구효질반흔형성급학습기억공능적영향. 방법 장96지성년SD대서안수궤수자표법분위공백대조조、등삼염수(NS)조화MMF조,매조32지.면역조화염색검측DAI후대서해마구활화소효질세포、활화성형효질세포이급류산연골소단백취당(CSPGs)적표체,Image-Pro Plus연건정량평고DAI역손구성형효질세포、소효질세포、CSPGs적표체량.Morris수미궁시험검측각조대서상후7～11d、28 ～ 32 d학습기억공능.대각조상후7,14,28 d효질반흔주요성분루적흡광도(IA)치급상후7～11d、28 ～ 32 d적Morris수미궁삼수진행통계분석,병대량자진행상관성분석. 결과 재각시상점,MMF조대서해마구효질반흔주요성분IA치균저우NS조여공백대조조(P＜0.05);재상후7～11d,각조대서Morris수미궁삼수(목표상한로정백분비화시간백분비)적차이무통계학의의차무임하상관성;재상후28 ～ 32 d,MMF조대서Morris수미궁삼수(목표상한로정백분비화시간백분비)명현강저(P＜0.01);상후28 d효질반흔적주요성분IA치여DAI대서28 ～32 d Morris수미궁삼수적평균속도화도닉잠복기정정상관관계,여목표상한정류로정백분비화정류시간백분비정부상관관계(P＜0.01). 결론 조기응용MMF능구현저억제DAI대서해마구효질반흔형성,개선DAI대서회복기적학습기억공능.
Objective To determine the intervention of mycophenolate mofetil (MMF) in glial scar formation and learning and memory function in a rat model of diffuse axonal injury (DAI).Methods Ninety-six SD rats were randomly divided into sham group, normal saline (NS) group and mycophenolate mofetil (MMF) group according to the random number table, with 32 rats per group.Immunohistochemistry was used to detect activated microglia cells, activated astrocytes and chondroitin sulphate proteoglycanns (CSPGs) in the hippocampus.Inage-Pro Plus software was used to quantitatively assess the changes of activated microglia cells, activated astrocytes and CSPGs.Morris water maze was applied for testing rat learning and memory function.Integrated absorbance (IA) of major constituents (microglia, astrosyte, chondroitin sulphate proteoglycan) of the glial scar was determined and analyzed for the correlation with the parameters of MWM.Results At 7, 14 and 28 days after injury, MMF group showed decreased IA of activated microglia in the hippocampus compared to sham and NS groups (P ＜ 0.05).At 7-11 days after injury, percent distance and percent time in the target quadrant of Morris water maze did not differ significantly among the three groups and were not related to the IA of glial scar.At 28-32 days after injury, percent distance and percent time in the target quadrant of Morris water maze lowered significantly in MMF group.At 28 days after injury, IA of the glial scar had a positive correlation with mean speed and mean escape latency, but negative correlation with percent distance and time in the target quadrant that measured in Morris water maze at 28-32 days after injury.Conclusion MMF significantly attenuates glial scar formation into the hippocampus and improves learning and memory function in rats during the recovery stage when administered in the early stage after DAI.
