CAJ | 학술논문

分别采用高效液相色谱/串联质谱法( HPLC-MS/MS )和高效液相色谱法( HPLC )对水产品中的诺氟沙星、环丙沙星和恩诺沙星喹诺酮类药物进行检测,旨在根据检测要求选择最佳的检测方法。通过分析检测限、线性范围、加标回收、精密度和方法比较2种检测方法,同时对22例市售水产品进行检测并比较分析。 HPLC-MS/MS法检测灵敏度高于HPLC法,且可快速、准确进行水产品中喹诺酮类药物检测。两种方法对恩诺沙星检测结果的相关系数为0．969,但HPLC检测数据低估于HPLC-MS/MS的2%。由于HPLC-MS/MS检测成本高,因此HPLC法可作为水产品中喹诺酮类药物残留检测的初步筛检方法,而HPLC -MS/MS法能准确定量水产品中喹诺酮类药物含量。
분별채용고효액상색보/천련질보법( HPLC-MS/MS )화고효액상색보법( HPLC )대수산품중적낙불사성、배병사성화은낙사성규낙동류약물진행검측,지재근거검측요구선택최가적검측방법。통과분석검측한、선성범위、가표회수、정밀도화방법비교2충검측방법,동시대22례시수수산품진행검측병비교분석。 HPLC-MS/MS법검측령민도고우HPLC법,차가쾌속、준학진행수산품중규낙동류약물검측。량충방법대은낙사성검측결과적상관계수위0．969,단HPLC검측수거저고우HPLC-MS/MS적2%。유우HPLC-MS/MS검측성본고,인차HPLC법가작위수산품중규낙동류약물잔류검측적초보사검방법,이HPLC -MS/MS법능준학정량수산품중규낙동류약물함량。
Quinolones which have demonstrated broad -spectrum activity against many pathogenic Gram -negative and Gram -positive bacteria are effective antibacterial drugs widely used against various important diseases of livestock and farmed fish. The European Union has established maximum residue limits ( MRL) for quinolone residues in animal tissues because of growing problem of microbial resistance. Thus, the estab-lishment of effective detection methods is required in order to control these drugs. Many papers have been published about the analysis of quinolone residues in animal products. High performance liquid chromatogra-phy/tandem mass spectrometry method ( HPLC - MS/MS ) and high performance liquid chromatography ( HPLC) method which are generally used were applied respectively to detect norfloxacin, ciprofloxacin and enrofloxacin in aquatic products because of their specificity, sensitivity, rapidity and robustness. The pur-pose of this paper was to select the suitable analytical method according to the requirement. The two methods had their own characteristics and were compared with regard to the detection limit, linear range, recovery, precision and influence factors of detection. Paired measurements were also applied to the analysis of commer-cially available 22 aquatic products. HPLC results were evaluated by comparison with the HPLC-MS/MS a-nalysis. The results showed that compared with HPLC, the detection sensitivity of HPLC-MS/MS was high-er. The use of HPLC-MS/MS could realize rapid, sensitive and accurate detection. HPLC results compared with HPLC-MS/MS data showed to underestimate enrofloxacin content of about 2% and a correlation coeffi-cient of 0. 969 was calculated between the two methods. HPLC method is simple and rapid, and its perform-ance could meet the requirements of the domestic and international legislation. HPLC analytical method which is provided for basic food safety inspection agencies in the routine analysis is suitable for preliminary screening of quinolones, while HPLC-MS/MS could be a more accurate quantitative determination method for measur-ing quinolones content in aquatic products for the reason that detailed structure information is provided by mass spectrometry.