CAJ | 학술논문

试验以杜洛克猪、大白猪、大围子猪、沙子岭猪、桃源黑猪、五指山猪和杜长大商品猪为试验材料,通过测序和PCR-RFLP的方法对胰岛素诱导基因2(INSIG2)5'调控区域的多态性进行检测,并分析该基因对猪肉质性状的影响,结果表明:在该区域发现T-502C、G-460C和T-609C 3个突变位点,T-502C和G-460C所选所有品种都处于Hardy-Weinberg平衡状态,T-609C除了五指山猪外也都符合Hardy-Weinberg平衡,在肉质性状上,T-502C位点,TT型个体的系水力和嫩度显著高于CC型;G-460C位点,杂合子CG型在贮存损失上显著高于CC型和GG型,大理石纹GG型和CG型差异不显著但是都显著高于CC型,肉质色度方面GG型和CC型差异不显著但显著高于CG型;T-609C位点,TT型的贮存损失显著高于CT型, 但和CC型差异不显著, 嫩度CC型显著高于CT型和TT型,INSIG2基因可能是一个与猪肉系水力、嫩度和大理石纹相关的分子遗传标记.
시험이두락극저、대백저、대위자저、사자령저、도원흑저、오지산저화두장대상품저위시험재료,통과측서화PCR-RFLP적방법대이도소유도기인2(INSIG2)5'조공구역적다태성진행검측,병분석해기인대저육질성상적영향,결과표명:재해구역발현T-502C、G-460C화T-609C 3개돌변위점,T-502C화G-460C소선소유품충도처우Hardy-Weinberg평형상태,T-609C제료오지산저외야도부합Hardy-Weinberg평형,재육질성상상,T-502C위점,TT형개체적계수력화눈도현저고우CC형;G-460C위점,잡합자CG형재저존손실상현저고우CC형화GG형,대리석문GG형화CG형차이불현저단시도현저고우CC형,육질색도방면GG형화CC형차이불현저단현저고우CG형;T-609C위점,TT형적저존손실현저고우CT형, 단화CC형차이불현저, 눈도CC형현저고우CT형화TT형,INSIG2기인가능시일개여저육계수력、눈도화대리석문상관적분자유전표기.
In this experiment, the Duroc, Yorkshire, Daweizi, Shaziling, Taoyuan, Wuzhishan and the DLY Hybrid were chose, the polymorphism of insulin induced genes 2 (INSIG2)'s 5' regulatory regionswere detected through the sequencing and PCR-RFLP method, and we also analyzed the association of polymorphisms of INSIG2 with the meat traits, the results show that this area has three mutations:T-502C, G-460C and T-609C, the T-502C and G-460C arein Hardy Weinberg equilibrium among all the selected varieties, T-609C are also in Hardy Weinberg equilibrium besides wuzhishan pigs; on the meat quality traits, for T-502C sites, water holding capacity and tenderness of TT type are significantly higher than that of CC type; for G-460C sites, CG type is significantly higher than CC and GG, in marble, GG and CG type was not significant but were significantly higher than that of CC, on fleshy chromaticity, GG type and CC was not significant, but significantly higher than CG type; for T-609C sites, TT is significantly higher than CT in storage loss, but no significant difference between CC type, in tenderness, CC was significantly higher than that of CT and TT, INSIG2 gene is likely to be one of the molecular genetic markers forwater holding capacity, tenderness and the marble.