CAJ | 학술논문

为确定脾毒保存期对猪睾丸细胞(Swine Testis, ST)繁殖猪瘟病毒的影响,选用不同保存期的脾毒接种ST细胞,培养猪瘟病毒,从感染率、产毒时间、病毒液效价、合格收次4个方面进行对比.结果显示,随着脾毒保存期延长,病毒对细胞感染率明显降低,产毒时间延后,所产病毒液效价下降,合格收次减少. 提示保存期对ST细胞产毒有很大影响,在生产中应严格控制脾毒种毒的保存期.
위학정비독보존기대저고환세포(Swine Testis, ST)번식저온병독적영향,선용불동보존기적비독접충ST세포,배양저온병독,종감염솔、산독시간、병독액효개、합격수차4개방면진행대비.결과현시,수착비독보존기연장,병독대세포감염솔명현강저,산독시간연후,소산병독액효개하강,합격수차감소. 제시보존기대ST세포산독유흔대영향,재생산중응엄격공제비독충독적보존기.
In order to determine the spleen-virus storage life influence on swine testicular cells to produce virus, we chose different storage life of spleen-virus to breed classical swine fever virus, compared four aspects: the infection rates of sells, begin time of virus to be produced, virus titer, harvest times. Results showed that, with the extension of spleen-virus storage life, the virus infection rates to sells significantly lower, virus-producing time delayed, virus titer decreased, harvest times reduced. Storage life of spleen-virus for ST cells to produce virus has a great influence, we should strictly control the spleen-virus storage life in the process of production.