CAJ | 학술논문

微生物批式流加发酵生产1,3-丙二醇是一种重要的生产方式,但是目前研究只考虑了细胞外5种物质浓度的变化,没有考虑代谢机理、细胞内物质浓度的变化及重要的中间产物.本文将细胞内甘油、3-羟基丙醛和1,3-丙二醇三种物质的浓度变化考虑在内,假设甘油跨膜运输情形为主动运输和被动扩散,1,3-丙二醇的跨膜运输情形为主动运输和被动扩散,建立了微生物批式流加发酵动力系统.以实验值与计算值的误差平方和作为性能指标建立参数辨识模型,并用改进的粒子群算法求解模型中参数.结果表明,改进后模型平均相对误差比现有文献略有降低,说明了模型的有效性.
미생물비식류가발효생산1,3-병이순시일충중요적생산방식,단시목전연구지고필료세포외5충물질농도적변화,몰유고필대사궤리、세포내물질농도적변화급중요적중간산물.본문장세포내감유、3-간기병철화1,3-병이순삼충물질적농도변화고필재내,가설감유과막운수정형위주동운수화피동확산,1,3-병이순적과막운수정형위주동운수화피동확산,건립료미생물비식류가발효동력계통.이실험치여계산치적오차평방화작위성능지표건립삼수변식모형,병용개진적입자군산법구해모형중삼수.결과표명,개진후모형평균상대오차비현유문헌략유강저,설명료모형적유효성.
The method of microbial fermentation is an important mode of production in producing 1,3-Propane-diol (1,3-PD). Although the microbial fed-batch culture of glycerol to 1,3-PD is a vital production mode, the present research results of microbial fed-batch culture system which only think about the concentration of five kinds extracellular materials changed instead of metabolic mechanism, concentration of intracellular materials changed and important intermediate products. We take the concentration of intracellular glycerol, 3-HPA and 1, 3-PD into consideration in this paper. A dynamic system of microbial fed-batch fermentation is built under the active transport and passive diffusion of glycerol and active transport and passive diffusion of 1,3-PD. Taking the sum of square error between experimental data and computational values as the performance index, the pa-rameter identification model is presented and an improved particle swarm optimization algorithm is developed to seek the unknown parameters in the model. Numerical simulations show that the average relative errors of biomass and glycerol have reduced slightly and illustrate the availability in the improved model.