CAJ | 학술논문

肉桂酰辅酶A还原酶（ Cinnamoyl－CoA reductase， CCR）是苯丙烷类代谢途径中的关键酶，在七彩红竹木质素和花青素合成代谢流向中起重要作用。依据七彩红竹转录组数据设计特异引物，采用反转录PCR技术从七彩红竹中克隆得到一个新的CCR基因的全长cDNA序列，命名为IhCCR－1（登录号： KP271440）。结果表明，该基因全长cDNA为1038 bp，编码345个氨基酸的蛋白质，属于酸性稳定蛋白，不存在信号肽，为非分泌蛋白；IhCCR－1蛋白具有保守的KNWYCYGK催化位点，属于NADB－Rossmann超家族，相对分子量为37．61 kDa； IhC－CR－1与其他植物的CCR具有较高的亲缘关系。研究结果将为进一步研究七彩红竹木质素产生的分子机理和综合开发利用奠定基础。
육계선보매A환원매（ Cinnamoyl－CoA reductase， CCR）시분병완류대사도경중적관건매，재칠채홍죽목질소화화청소합성대사류향중기중요작용。의거칠채홍죽전록조수거설계특이인물，채용반전록PCR기술종칠채홍죽중극륭득도일개신적CCR기인적전장cDNA서렬，명명위IhCCR－1（등록호： KP271440）。결과표명，해기인전장cDNA위1038 bp，편마345개안기산적단백질，속우산성은정단백，불존재신호태，위비분비단백；IhCCR－1단백구유보수적KNWYCYGK최화위점，속우NADB－Rossmann초가족，상대분자량위37．61 kDa； IhC－CR－1여기타식물적CCR구유교고적친연관계。연구결과장위진일보연구칠채홍죽목질소산생적분자궤리화종합개발이용전정기출。
Cinnamoyl-CoA reductase ( CCR) is a key enzyme in the phenylpropanoid pathway, and plays a critical role in metabolic flow of lignin and anthocyanin.In this study, we cloned the full-length of cDNA of a novel CCR gene from Indosasa hispida cv.Rainbow with the method of reverse transcription PCR and gene-specific primers u-sing a semi-quantitative RT-PCR technique according to the transcriptome sequencing data.This novel gene was named as IhCCR-1 ( Accession Number: KP271440 ) .It has a length of 1 038 bp and encoded with a protein of 345 amino acids.The IhCCR-1 protein is a stable acidic and non-secretory protein that has no signalpeptide.The IhCCR-1 protein belonged to the NADB-Rossmann superfamily and contained the conserved KNWYCYGK motif. IhCCR-1 is closely related to the other plants.The results above would lay a theoretical basis for further exploration of molecular mechanism of lignin and for the comprehensive exploitation and utilization of Indosasa hispida cv.Rainbow.