CAJ | 학술논문

以三球悬铃木成熟果球为材料，研究不同消毒方式与不同无机盐浓度对其种子萌发的影响，筛选三球悬铃木果球离体培养的最适培养基；以无菌幼苗叶片为外植体，探讨叶龄、光照条件对愈伤组织诱导及植物生长调节剂浓度对不定芽分化的影响，建立高效的叶片再生体系。结果表明，三球悬铃木成熟种子经10％双氧水溶液（ H2 O2）消毒处理10 min，在EX培养基上培养，可显著提高其种子萌发率，获得大量无菌组培苗；然后将叶龄40天的无菌苗叶片，经7天暗培养处理后转到光照下继续诱愈培养，愈伤组织诱导率可达到89．33％；再接种于分化培养基MS＋IBA 0．1 mg／L＋KT 1．0 mg／L＋6－BA 1．5 mg／L中，经30天光照培养，不定芽分化率可达66．67％。
이삼구현령목성숙과구위재료，연구불동소독방식여불동무궤염농도대기충자맹발적영향，사선삼구현령목과구리체배양적최괄배양기；이무균유묘협편위외식체，탐토협령、광조조건대유상조직유도급식물생장조절제농도대불정아분화적영향，건립고효적협편재생체계。결과표명，삼구현령목성숙충자경10％쌍양수용액（ H2 O2）소독처리10 min，재EX배양기상배양，가현저제고기충자맹발솔，획득대량무균조배묘；연후장협령40천적무균묘협편，경7천암배양처리후전도광조하계속유유배양，유상조직유도솔가체도89．33％；재접충우분화배양기MS＋IBA 0．1 mg／L＋KT 1．0 mg／L＋6－BA 1．5 mg／L중，경30천광조배양，불정아분화솔가체66．67％。
In vitro procedure for shoot regeneration of Plantanus orientalis was set up using mature fruit-seeds as starting materials.Several factors including disinfection method, explant age, and light were investigated to optimize the regeneration system.The results showed that the seeds treated with 10%hydrogen peroxide (H2 O2 ) for 10 min, could germinate effectively on EX medium.The robust explants were those 40-day leaves, and the cultured plantlets could be used as explants to induce a high frequency of callus at about 89.33%in dark for 7 d followed with light treatment, and a high frequency of shoot differentiation more than 66.67 %on MS medium supplemented with IBA 0.1 mg/L, KT 1.0 mg/L and 6-BA 1.5 mg/L could be obtained with 30 d light treatment.