CAJ | 학술논문

将从循环水养殖系统中收集的新吉富罗非鱼粪便，置于异位式生物絮凝反应器中，用显微镜和扫描电镜观察了粪便转化为絮体过程中结构变化，并测定了粗蛋白、氨基酸、胞外聚合物的变化。粪便在转化过程中结构变疏松，直至最终发展成以丝状菌为骨架的生物絮体。随着粪便的分解，自第4d开始，反应器中氨氮含量开始缓慢上升，第7d达到峰值7．46mg／L，亚硝态氮也随之升至7．81mg／L，并且硝态氮也开始快速增至21．6 m g／L。絮体粗蛋白含量在第9 d达到最高值（26．37％），总氨基酸在第7 d达到最高值20．44％。絮体中的总氨基酸含量虽比粪便高，但比饲料中总氨基酸33．16％低。絮体疏松结合的胞外聚合物的主要成分是多糖，而紧密结合的胞外聚合物中蛋白质是其主要成分。
장종순배수양식계통중수집적신길부라비어분편，치우이위식생물서응반응기중，용현미경화소묘전경관찰료분편전화위서체과정중결구변화，병측정료조단백、안기산、포외취합물적변화。분편재전화과정중결구변소송，직지최종발전성이사상균위골가적생물서체。수착분편적분해，자제4d개시，반응기중안담함량개시완만상승，제7d체도봉치7．46mg／L，아초태담야수지승지7．81mg／L，병차초태담야개시쾌속증지21．6 m g／L。서체조단백함량재제9 d체도최고치（26．37％），총안기산재제7 d체도최고치20．44％。서체중적총안기산함량수비분편고，단비사료중총안기산33．16％저。서체소송결합적포외취합물적주요성분시다당，이긴밀결합적포외취합물중단백질시기주요성분。
The bioflocculation process in a reactor was observed under a microscope and scanning electronic microscope and crude protein ,amino acids and extracellular polymeric substances were determined in feces of NEW GIFT Oreochromis niloticus collected in a recirculating aquaculture system .It was found that the feces became loosely ,feces developed into bioflocs framed with filamentous fungi with reaction of the bioflocs .With the decomposition of feces ,there was slow increase in level of the ammonium ,with the peak value of 7 .46 mg/L in the reactor on the 7th day ,and the nitrite was shown to be increased to 7 .81 mg/L , with the rapidly accumulative nitrate of 21 .6 mg/L .On the 9th day ,the crude protein had the peak content of 26 .37% ,and then decreased to 25 .45% ,and the total amino acids was found to be the peak value of 20 .44% on the 7th day ,and then decreased to 18 .11% .There were lower level of essential amino acids in bioflocs than in the feed (13 .03% ) , even though higher than in the feces (9 .36% ) . The polysaccharides were primariy substance in the loosely bound extracellular polymeric substances(LB‐EPS) in the bioflocs ,while protein was the main component in the tightly bound EPS(TB‐EPS)in the bioflocs .