中国民族民间医药
中國民族民間醫藥
중국민족민간의약
Chinese Journal of Ethnomedicine and Ethnopharmacy
2015年
18期
20-22
,共3页
乙肝宁复方%多糖%苯酚 -硫酸法%除蛋白
乙肝寧複方%多糖%苯酚 -硫痠法%除蛋白
을간저복방%다당%분분 -류산법%제단백
compound Yiganning%polysaccharide%phenol -sulfuric acid method%removing protein
目的：对乙肝宁复方多糖进行三种除蛋白处理，考查除蛋白效果。方法：采用苯酚－硫酸法在490 nm 处测定糖的含量，以多糖回收率和蛋白去除率为指标。结果：Sevag 法、5％三氯乙酸法、三氯乙酸－正丁醇法的多糖回收率分别为57．41％、88．27％、64．90％，蛋白去除率分别为53．06％、20．23％、28．90％。结论：5％三氯乙酸法对多糖的保留效果最好，Sevag 法除蛋白效果最好，本实验为多糖除蛋白的方法提供了实验依据。
目的：對乙肝寧複方多糖進行三種除蛋白處理，攷查除蛋白效果。方法：採用苯酚－硫痠法在490 nm 處測定糖的含量，以多糖迴收率和蛋白去除率為指標。結果：Sevag 法、5％三氯乙痠法、三氯乙痠－正丁醇法的多糖迴收率分彆為57．41％、88．27％、64．90％，蛋白去除率分彆為53．06％、20．23％、28．90％。結論：5％三氯乙痠法對多糖的保留效果最好，Sevag 法除蛋白效果最好，本實驗為多糖除蛋白的方法提供瞭實驗依據。
목적：대을간저복방다당진행삼충제단백처리，고사제단백효과。방법：채용분분－류산법재490 nm 처측정당적함량，이다당회수솔화단백거제솔위지표。결과：Sevag 법、5％삼록을산법、삼록을산－정정순법적다당회수솔분별위57．41％、88．27％、64．90％，단백거제솔분별위53．06％、20．23％、28．90％。결론：5％삼록을산법대다당적보류효과최호，Sevag 법제단백효과최호，본실험위다당제단백적방법제공료실험의거。
Objective In order to test effect to three methods to removing protein of compound polysaccharides in Yiganning. Methods The polysaccharide content was determined by phenol -sulfuric acid method comparing colors at 490 nm .With polysaccha-ride recovery rate and protein removal rate as an indicator.Results Sevag method,5% TCA method ,TCA -Butanol method of poly-saccharide recovery rates were 57.41%,88.27%,64.90%,while protein removal rates were 53.06%,20.23%,28.90%.Con-clusion 5% TCA method on polysaccharide retention effect is better,Sevag method protein removal effect is better.The experiment provides basis for the study of removing protein method.
