CAJ | 학술논문

为了提高山核桃仁鞣质的肿瘤靶向性,增强其抗肿瘤活性,采用薄膜分散法制备山核桃仁鞣质叶酸-PEG修饰纳米粒,通过核磁共振、透射电镜等方法对纳米粒的结构、粒径和粒径分布、形态、稳定性、包封率以及肿瘤靶向性进行了初步研究.结果表明,山核桃仁鞣质叶酸-PEG修饰纳米粒平均粒径为166．8 nm；包封率为74．5％,粒子呈大小均匀的球形；放置50 d纳米粒粒径和包封率变化轻微,具有很好的稳定性；叶酸-PEG 修饰纳米粒小鼠尾静脉注射后0．25,1和4 h 肿瘤分布分别为20．8％,36．2％和50．2％,具备明显的肿瘤靶向作用.
위료제고산핵도인유질적종류파향성,증강기항종류활성,채용박막분산법제비산핵도인유질협산-PEG수식납미립,통과핵자공진、투사전경등방법대납미립적결구、립경화립경분포、형태、은정성、포봉솔이급종류파향성진행료초보연구.결과표명,산핵도인유질협산-PEG수식납미립평균립경위166．8 nm；포봉솔위74．5％,입자정대소균균적구형；방치50 d납미립립경화포봉솔변화경미,구유흔호적은정성；협산-PEG 수식납미립소서미정맥주사후0．25,1화4 h 종류분포분별위20．8％,36．2％화50．2％,구비명현적종류파향작용.
In order to enhance the tumor-targeting and anticancer activity of tannins in walnut,tannin-loaded nanoparticles which decorated by FA-PEG were prepared through film dispersion method.1 H-NMR was em-ployed to characterize the structure of FA-PEG-NH2 .The transmitting electron microscope was characterized the morphology of nanoparticles.The average diameter of nanoparticles and size distribution were determined by dynamic light scattering.The drug encapsulation efficiency and tumor-targeting characteristics were determined by HPLC.The results indicated that the nanoparticles were uniform spherical and regular.Its average size was 166.8 nm,and encapsulation efficiency was 74.5%.The size and encapsulation efficiency stability of nanoparti-cles was good because they were little change after 50 d.When the time was 0.25,1,4 h after nanoparticles were injected in tail vein of mice,the distribution of drug concentrations in tumor respectively was 20.8%, 36.2%,50.2%.The tumor targeting characteristics of nanoparticles were obvious.