CAJ | 학술논문

为研究复合益生菌对草鱼( Ctenopharyngodon idellus)生长和抗氧化功能的影响, 实验设1个对照组( (Ⅰ组)投喂基础饲料)和3个实验组(投喂在基础饲料中添加复合益生菌的饲料, 添加浓度分别为1000 mg/kg (Ⅱ组)、2000 mg/kg(Ⅲ组)和3000 mg/kg(Ⅳ组)), 每组3个重复, 饲养初始体重为(175. 79 ± 15. 32)g草鱼8周后, 测定生长和抗氧化指标. 结果显示, 各实验组均能显著提高草鱼增重率、特定生长率, 降低饵料系数, 存活率无显著差异, 且Ⅲ组增重率和特定生长率最高, 饵料系数最低, 增重率比Ⅰ组提高了16. 32%. 复合益生菌显著提高了草鱼肠道淀粉酶、脂肪酶和胰蛋白酶活性, 其中Ⅲ组的脂肪酶和胰蛋白酶活性最高, Ⅳ组的淀粉酶活性最高;复合益生菌显著提高了草鱼血清中总抗氧化能力、过氧化氢酶、超氧化物歧化酶、谷胱甘肽过氧化物酶活性和谷胱甘肽含量, 当添加量为3000 mg/kg时, 会降低其超氧化物歧化酶活性; 复合益生菌能显著降低草鱼血清中抗超氧阴离子自由基活性和丙二醛含量, 当添加量为3000 mg/kg时, 抗超氧阴离子自由基活性和丙二醛含量均有所升高. 结果表明草鱼基础饲料中适量添加复合益生菌能促进其生长, 降低饵料系数, 提高肠道消化酶活性和抗氧化功能, 适宜添加量为2000 mg/kg.
위연구복합익생균대초어( Ctenopharyngodon idellus)생장화항양화공능적영향, 실험설1개대조조( (Ⅰ조)투위기출사료)화3개실험조(투위재기출사료중첨가복합익생균적사료, 첨가농도분별위1000 mg/kg (Ⅱ조)、2000 mg/kg(Ⅲ조)화3000 mg/kg(Ⅳ조)), 매조3개중복, 사양초시체중위(175. 79 ± 15. 32)g초어8주후, 측정생장화항양화지표. 결과현시, 각실험조균능현저제고초어증중솔、 특정생장솔, 강저이료계수, 존활솔무현저차이, 차Ⅲ조증중솔화특정생장솔최고, 이료계수최저, 증중솔비Ⅰ조제고료16. 32%. 복합익생균현저제고료초어장도정분매、 지방매화이단백매활성, 기중Ⅲ조적지방매화이단백매활성최고, Ⅳ조적정분매활성최고;복합익생균현저제고료초어혈청중총항양화능력、 과양화경매、 초양화물기화매、 곡광감태과양화물매활성화곡광감태함량, 당첨가량위3000 mg/kg시, 회강저기초양화물기화매활성; 복합익생균능현저강저초어혈청중항초양음리자자유기활성화병이철함량, 당첨가량위3000 mg/kg시, 항초양음리자자유기활성화병이철함량균유소승고. 결과표명초어기출사료중괄량첨가복합익생균능촉진기생장, 강저이료계수, 제고장도소화매활성화항양화공능, 괄의첨가량위2000 mg/kg.
An 8 weeks experiment was conducted to evaluate the effect of the compound probiotics on growth performance and digestive enzyme activities and antioxidant function in Ctenopharyngodon idellus ( initial average body weight was 175. 79 ±15. 32 g). We added compound probiotics 0 mg/kg (control groupⅠ), 1000 mg/kg (group Ⅱ), 2000 mg/kg ( groupⅢ) and 3000 mg/kg ( groupⅣ) to the basal diet of Ctenopharyngodon idellus, respectively. The results showed as follows: weight gain rate ( WGR) and specific growth rate ( SGR) of the experiment groups increased significantly than the control group, while survival rate had no significant differences. Weight gain in Ⅲ group increased 16. 32% compared to that in groupⅠ. The activities of digestive enzymes in intestine and total antioxidant capacity ( T - AOC ) , catalase ( CAT) , superoxide dismutase ( SOD) , glutathione peroxidase ( GSH-PX) activity and glutathione ( GSH) levels signif-icantly increased by adding compound probiotics, but the activity of superoxide dismutase ( SOD) decreased when exces-sive add (3000 mg/kg) compound probiotics in Ctenopharyngodon idellus. The activities of anti-superoxide-anion free radical ( ASAFR) and malondialdehyde ( MDA) levels significantly reduced by adding compound probiotics in serum, but the opposite effect was performanced when excessive add of compound probiotics in Ctenopharyngodon idellus. Based on these observations above, it was concluded that the growth performance and the activities of digestive enzymes and antioxi-dant capacity of Ctenopharyngodon idellus could be improved by adding compound probiotics, and the appropriate adding level of compound probiotics was 2000 mg/kg.