CAJ | 학술논문

基于Tween-80能增敏富勒醇(F-ol)的荧光信号剧烈增强，以及F-ol中的-OH与细胞色素C (Cyt C)分子中的 HOOC-反应生成无荧光化合物而导致F-ol的荧光信号剧烈猝灭,设计了一种测定痕量Cyt C新的荧光探针.本方法灵敏（检出限为2.3×10-17g mL-1），选择性高(Er≤±5℅,无共存物的干扰)、精密度好（RSD%为2.1-2.5）、准确，用于人血清中痕量Cyt C的测定和人体疾病的诊断，结果与CdTe/CdS 量子点共振瑞利散射光谱法相吻合，同时探讨了新方法测定痕量Cyt C的反应机理.
기우Tween-80능증민부륵순(F-ol)적형광신호극렬증강，이급F-ol중적-OH여세포색소C (Cyt C)분자중적 HOOC-반응생성무형광화합물이도치F-ol적형광신호극렬졸멸,설계료일충측정흔량Cyt C신적형광탐침.본방법령민（검출한위2.3×10-17g mL-1），선택성고(Er≤±5℅,무공존물적간우)、정밀도호（RSD%위2.1-2.5）、준학，용우인혈청중흔량Cyt C적측정화인체질병적진단，결과여CdTe/CdS 양자점공진서리산사광보법상문합，동시탐토료신방법측정흔량Cyt C적반응궤리.
A new fluorescence probe for the determination of trace Cytochrome C (Cyt C) has been established, based on signal magnification effect of Tween-80 on fullerol (F-ol) fluorescent intensity and the reactivity of the active -OH group in F-ol with the-COOH group of Cyt C molecular to form an ( F-ol)2-Cyt C-Tween-80 no fluorescent compounds to cause the severe quenching of fluorescent signal. This method had high sensitivity(The detection limit:2.3 × 10-17g mL-1), good selecti-vity (The relative error≤± 5℅, coexistence species were of no interference), high precision (The relative standard deviation was 2.1 %-2.5 %) and accuracy. It was applied to the determination of trace Cyt C in human serum and the diagnosis of human diseases. The results were tallied with those of quantum dots resonance Rayleigh scattering spectroscopy. The mechanism of new fluorescence quenching method for the determination of trace Cyt C was discussed, which laid the theory foundation for the analytical application of F-ol in life science.