CAJ | 학술논문

海藻酸钙微胶珠具有良好的生物相容性、可降解、机械强度高、韧性好等优点,已被广泛应用于组织工程研究.骨粉中含有大量的羟基磷灰石及 BMP等生长因子,具有良好的骨诱导活性.结合上述两种材料的优点,制备了一种复合海藻酸钙/骨粉微胶珠,并系统研究了人源脂肪干细胞(adipose-derived stem cells,ADSCs)在其中的最佳包埋密度.通过正交实验综合考虑海藻酸钠溶液浓度、氯化钙溶液浓度和骨粉密度对海藻酸钙/骨粉微胶珠机械强度及传质性能的影响,并采用液滴法制备了最优化的用于ADSCs 体外增殖分化的微胶珠.结果表明,海藻酸钠溶液的浓度对微胶珠传质能力和机械强度的影响最大,CaCl2溶液浓度其次,骨粉密度的影响最小.当海藻酸钠溶液浓度、CaCl2溶液浓度和骨粉密度分别为2．5％,4．5％和5．0 mg/mL时,微胶珠的性能最好.对细胞扩增倍数的比较和死活染色等检测表明,当ADSCs的初始包埋密度为5×106 cells/mL 时,更适于 ADSCs 的扩增与分化.
해조산개미효주구유량호적생물상용성、가강해、궤계강도고、인성호등우점,이피엄범응용우조직공정연구.골분중함유대량적간기린회석급 BMP등생장인자,구유량호적골유도활성.결합상술량충재료적우점,제비료일충복합해조산개/골분미효주,병계통연구료인원지방간세포(adipose-derived stem cells,ADSCs)재기중적최가포매밀도.통과정교실험종합고필해조산납용액농도、록화개용액농도화골분밀도대해조산개/골분미효주궤계강도급전질성능적영향,병채용액적법제비료최우화적용우ADSCs 체외증식분화적미효주.결과표명,해조산납용액적농도대미효주전질능력화궤계강도적영향최대,CaCl2용액농도기차,골분밀도적영향최소.당해조산납용액농도、CaCl2용액농도화골분밀도분별위2．5％,4．5％화5．0 mg/mL시,미효주적성능최호.대세포확증배수적비교화사활염색등검측표명,당ADSCs적초시포매밀도위5×106 cells/mL 시,경괄우 ADSCs 적확증여분화.
Calcium alginate/bone-powder microbeads have been widely used in tissue engineering research due to their excellent biocompatibility,biodegradability,enhanced mechanical strength and toughness.Bone-powder containing abundant hydroxylapatite and growth factors such as BMP,possess an excellent osteoinductive activ-ity.Herein,a hybrid calcium alginate/bone-powder microbeads combining both advantages of the abovemen-tioned materials was prepared,afterwards the optimal inoculating density of the Adipose-derived stem cells (ADSCs)embedded in hybrid microbeads was explored systematically.The optimized microbeads suitable to the in vitro expansion and differentiation of ADSCs,were prepared using the droplet method under overall consider-ing the concentration of calcium alginate and calcium chloride,the density of bone powder,also by the orthogo-nal experiment.It demonstrates that the concentration of the sodium alginate has the strongest influence on the mass transfer and mechanical strength of the microbeads,secondly the calcium chloride,then the density of bone powder.The microbeads perform best when the concentrations of the sodium alginate and calcium chloride are 2.5% and 4.5% respectively,as well as the density of bone powder was 5.0 mg/mL.Additionally,the com-parison about cell expanding fold and live/dead staining are carried out,which shows the ADSCs expand and differentiate well with the initial embedding density of 5×106 cells/mL.