CAJ | 학술논문

目的：探讨控制性皮质撞击(CCI)法建立大鼠脑外伤模型的昏迷、神经行为学和认知功能表现。方法40只雄性Sprague-Dawley大鼠随机分成对照组(n=10)、假手术组(n=10)、CCI组(n=20)。CCI组大鼠进行双侧额叶打击，打击速度3.5 m/s，深度7 mm，停留时间250 ms。记录昏迷时间。于术后10 d、20 d分别进行水迷宫测试。结果 CCI组术后意识障碍时间较对照组和假手术组延长(P<0.05)。术后10 d、20 d，CCI组逃避潜伏期均较对照组和假手术组显著延长(P<0.001)，目标象限停留时间百分比显著减少(P<0.001)。结论大鼠双侧额叶CCI可以建立重度脑外伤昏迷动物模型，对认知障碍产生长期影响。
목적：탐토공제성피질당격(CCI)법건립대서뇌외상모형적혼미、신경행위학화인지공능표현。방법40지웅성Sprague-Dawley대서수궤분성대조조(n=10)、가수술조(n=10)、CCI조(n=20)。CCI조대서진행쌍측액협타격，타격속도3.5 m/s，심도7 mm，정류시간250 ms。기록혼미시간。우술후10 d、20 d분별진행수미궁측시。결과 CCI조술후의식장애시간교대조조화가수술조연장(P<0.05)。술후10 d、20 d，CCI조도피잠복기균교대조조화가수술조현저연장(P<0.001)，목표상한정류시간백분비현저감소(P<0.001)。결론대서쌍측액협CCI가이건립중도뇌외상혼미동물모형，대인지장애산생장기영향。
Objective To establish the model of traumatic brain injury in rats with controlled cortical impact (CCI), and investigate the features of coma and cognition. Methods 40 male Sprague-Dawley rats were randomly divided into control group (n=10), sham group (n=10) and CCI group (n=20). CCI group was impacted at bilateral frontal lobe with the velocity of 3.5 m/s, depth of 7 mm and dwell time of 250 ms. The time of consciousness loss was recorded, and all the rats were investigated with Morris Water Maze 10 and 20 days after opera-tion. Results The time of consciousness loss was longer in the CCI group than in the control group and the sham group (P<0.001). The es-cape latency was longer in the CCI group than in the control group and the sham group (P<0.001), while the percent of time spend in goal quarter during probe trial was less (P<0.001), both 10 days and 20 days after operation. Conclusion CCI at bilateral frontal lobe of rats can establish the model of severe traumatic brain injury with coma and long-term cognitive dysfunction.