中国康复理论与实践
中國康複理論與實踐
중국강복이론여실천
Chinese Journal of Rehabilitation Theory and Practice
2015年
10期
1156-1161
,共6页
荆瀛黎%刘小野%白帆%陈惠
荊瀛黎%劉小野%白帆%陳惠
형영려%류소야%백범%진혜
脊髓损伤%血脊髓屏障%基质金属蛋白酶-9%紧密连接蛋白%小鼠
脊髓損傷%血脊髓屏障%基質金屬蛋白酶-9%緊密連接蛋白%小鼠
척수손상%혈척수병장%기질금속단백매-9%긴밀련접단백%소서
spinal cord injury%blood-spinal cord barrier%matrix metalloproteinases-9%occludin%mice
目的：探讨脊髓损伤后基质金属蛋白酶-9(MMP-9)表达变化对血脊髓屏障(BSCB)通透性的影响。方法 C57BL/6小鼠(n=68)随机分为假手术组、损伤后2 d组(S2)、损伤后7 d组(S7)和损伤后14 d组(S14)，每组17只。损伤组采用改良Allen's法制作中度脊髓损伤模型。在损伤后的第2、7、14天分别对小鼠进行盲法的小鼠后肢运动功能(BMS)评分和BMS亚评分；各组小鼠腹腔注入伊文思蓝，检测BSCB通透性；采用免疫荧光法检测紧密连接蛋白(occludin)表达，采用免疫印迹法检测occludin和MMP-9表达。结果术后2 d BMS评分显著降低，BSCB通透性显著增高；术后14 d BMS评分升高(P<0.01)，BSCB通透性下降(P<0.05)。术后7 d，occludin表达下调，MMP-9表达上调(P<0.05)；术后14 d occludin表达上调，MMP-9表达下调(P<0.05)。结论脊髓损伤后MMP-9可能通过介导occludin表达，影响BSCB通透性改变。
目的：探討脊髓損傷後基質金屬蛋白酶-9(MMP-9)錶達變化對血脊髓屏障(BSCB)通透性的影響。方法 C57BL/6小鼠(n=68)隨機分為假手術組、損傷後2 d組(S2)、損傷後7 d組(S7)和損傷後14 d組(S14)，每組17隻。損傷組採用改良Allen's法製作中度脊髓損傷模型。在損傷後的第2、7、14天分彆對小鼠進行盲法的小鼠後肢運動功能(BMS)評分和BMS亞評分；各組小鼠腹腔註入伊文思藍，檢測BSCB通透性；採用免疫熒光法檢測緊密連接蛋白(occludin)錶達，採用免疫印跡法檢測occludin和MMP-9錶達。結果術後2 d BMS評分顯著降低，BSCB通透性顯著增高；術後14 d BMS評分升高(P<0.01)，BSCB通透性下降(P<0.05)。術後7 d，occludin錶達下調，MMP-9錶達上調(P<0.05)；術後14 d occludin錶達上調，MMP-9錶達下調(P<0.05)。結論脊髓損傷後MMP-9可能通過介導occludin錶達，影響BSCB通透性改變。
목적：탐토척수손상후기질금속단백매-9(MMP-9)표체변화대혈척수병장(BSCB)통투성적영향。방법 C57BL/6소서(n=68)수궤분위가수술조、손상후2 d조(S2)、손상후7 d조(S7)화손상후14 d조(S14)，매조17지。손상조채용개량Allen's법제작중도척수손상모형。재손상후적제2、7、14천분별대소서진행맹법적소서후지운동공능(BMS)평분화BMS아평분；각조소서복강주입이문사람，검측BSCB통투성；채용면역형광법검측긴밀련접단백(occludin)표체，채용면역인적법검측occludin화MMP-9표체。결과술후2 d BMS평분현저강저，BSCB통투성현저증고；술후14 d BMS평분승고(P<0.01)，BSCB통투성하강(P<0.05)。술후7 d，occludin표체하조，MMP-9표체상조(P<0.05)；술후14 d occludin표체상조，MMP-9표체하조(P<0.05)。결론척수손상후MMP-9가능통과개도occludin표체，영향BSCB통투성개변。
Objective To investigate the influence of matrix metalloproteinases-9 (MMP-9) on permeability of blood-spinal cord barrier (BSCB) after spinal cord injury (SCI). Methods 68 male C57BL/6 mice were randomly divided into sham group, 2 days group (S2), 7 days group (S7) and 14 days group (S14) after SCI with 17 mice in each group. All the groups received a moderate impacted spinal cord injury ex-cept the sham group. Evan's Blue (EB) was administered intraperitoneally to detect the permeability of BSCB. Occludin was analyzed by im-munofluorescence, the expressions of occludin and MMP-9 were detected by Western blotting. Results After SCI, BMS score significantly reduced, compared with S2 group, S14 group showed a significant increase (P<0.01). The permeability of BSCB was seriously damaged af-ter SCI. Compared with S2 group, S14 group showed a notable down-regulation in the permeability of injured micro-vessels (P<0.05). The expression of occludin was down-regulated and the expression of MMP-9 was up-regulated 7 days after SCI (P<0.05). Compared with S2 group, S14 group showed a significant up-regulation of occludin and a remarkable down-regulation of MMP-9 (P<0.05). Conclusion After SCI, MMP-9 might mediate the expression of occludin to influence the BSCB permeability.
