CAJ | 학술논문

目的：探讨针康法对局灶性脑缺血大鼠神经功能缺损及缺血区碱性成纤维细胞生长因子(bFGF)和血管抑制素(AS)蛋白表达的影响。方法采用随机数字表法将90只清洁健康级雄性Sprague-Dawley大鼠随机分为假手术组、模型组、针刺治疗组、康复治疗组和针康治疗组，各组又分为3 d、7 d、14 d 3个亚组(n=6)。改良Longa法制备永久性脑缺血大鼠模型。模型组与假手术组不予任何治疗，针刺治疗组采用头穴丛刺疗法，康复治疗组采用跑台训练，针康治疗组采用针康法治疗。术后3d、7d、14d采用改良神经功能缺损评分(mNSS)评估各实验组大鼠的神经功能，Western blotting法检测各实验组大鼠缺血半暗区bFGF和AS蛋白的表达。结果术后3 d、7 d、14 d，与模型组、针刺治疗组、康复治疗组比较，针康治疗组mNSS评分降低(P<0.05)，bFGF蛋白表达升高(P<0.05)， AS蛋白表达降低(P<0.05)。结论针康法能降低局灶性脑缺血大鼠神经功能缺损，其机制可能与上调bFGF蛋白表达、下调AS蛋白表达有关。
목적：탐토침강법대국조성뇌결혈대서신경공능결손급결혈구감성성섬유세포생장인자(bFGF)화혈관억제소(AS)단백표체적영향。방법채용수궤수자표법장90지청길건강급웅성Sprague-Dawley대서수궤분위가수술조、모형조、침자치료조、강복치료조화침강치료조，각조우분위3 d、7 d、14 d 3개아조(n=6)。개량Longa법제비영구성뇌결혈대서모형。모형조여가수술조불여임하치료，침자치료조채용두혈총자요법，강복치료조채용포태훈련，침강치료조채용침강법치료。술후3d、7d、14d채용개량신경공능결손평분(mNSS)평고각실험조대서적신경공능，Western blotting법검측각실험조대서결혈반암구bFGF화AS단백적표체。결과술후3 d、7 d、14 d，여모형조、침자치료조、강복치료조비교，침강치료조mNSS평분강저(P<0.05)，bFGF단백표체승고(P<0.05)， AS단백표체강저(P<0.05)。결론침강법능강저국조성뇌결혈대서신경공능결손，기궤제가능여상조bFGF단백표체、하조AS단백표체유관。
Objective To study the effect of cluster needling of scalp acupuncture combined with rehabilitation on neurological function and expression of basic fibroblast growth factor (bFGF) and angiostatin (AS) in rats after focal cerebral ischemia. Methods 90 male Sprague-Dawley rats were randomly divided into sham operation group, model group, acupuncture treatment group, rehabilitation treatment group and acupuncture and rehabilitation treatment group, each group was further divided into 3 days, 7 days and 14 days subgroups (n=6). Permanent cerebral ischemia rat model was established according to Longa's method. The model group and the sham operation group accept-ed no treatment, the acupuncture treatment group was treated by cluster needling of scalp acupuncture, the rehabilitation treatment group re-ceived the treadmill training, the acupuncture and rehabilitation treatment group was treated by acupuncture and rehabilitation therapy. 3 days, 7 days, 14 days after operation, the neural function was evaluated with the modified Neurological Severity Score (mNSS), the expres-sion levels of bFGF and AS proteins were detected by Western blotting. Results Compared with the model group, the acupuncture treatment group and the rehabilitation treatment group, mNSS decreased, the expression of bFGF protein increased, and the expression of AS protein decreased in the acupuncture and rehabilitation treatment group 3 days, 7 days, 14 days after operation (P<0.05). Conclusion Acupuncture and rehabilitation therapy can reduce the neurological function defect in rats with focal cerebral ischemia, which may be related to the up-regulation of bFGF protein expression and down-regulation of AS protein expression.