CAJ | 학술논문

目的：观察针刺对抑郁症睡眠障碍（SDD）大鼠海马组织及血清中 SOD 活性和 MDA 含量的影响。方法正常组8只 SD 大鼠常规合笼饲养。32只 SD 大鼠接受为期21 d 的孤养＋ CUMS 法＋ REM 睡眠剥夺法造模，其中28只完成造模，二次分组为模型组、针刺组、药物组、针药组，每组各7只。模型组仅造模不干预；针刺组取印堂、神庭、太冲（双）进行针刺；药物组给予舍曲林和阿普唑仑水溶液灌胃；针药组同时结合针刺组与药物组的两种干预方式；3组均连续干预7 d。结果 SDD 造模致大鼠海马组织〔（41.19±5.59）u/ mL〕和血清〔（34.57±5.53）u/ mL〕的 SOD 活性较正常组〔（60.19±8.85）、（56.20±13.68）u/ mL〕明显下调（P ＜0.05），且致海马组织〔（5.12±1.22）nmol/ mg〕和血清〔（6.22±0.96）nmol/ mg〕的 MDA 含量较正常组〔（3.49±1.02）、（4.35±1.55）nmol/ mg〕明显上调（P ＜0.05）；针刺组海马组织〔（52.29±11.37）u/ mL〕和血清〔（54.32±6.95）u/ mL〕SOD 活性均较模型组有明显的上调（P ＜0.05），且仅针刺组的海马组织〔（3.86±0.80）nmol/ mg〕MDA 含量较模型组得到了明显的下调（P ＜0.05）；药物组仅上调了血清 SOD 活性〔（44.70±5.31）u/ mL，P ＜0.05）〕；针药组上调了海马组织〔（51.95±7.58）u/ mL〕和血清〔（48.23±6.70）u/ mL〕的 SOD 活性（P ＜0.05）。结论 SDD 造模能明显加速衰老；3种干预手段均有一定的抗衰老作用，其中针刺组最优，针药组次之。
목적：관찰침자대억욱증수면장애（SDD）대서해마조직급혈청중 SOD 활성화 MDA 함량적영향。방법정상조8지 SD 대서상규합롱사양。32지 SD 대서접수위기21 d 적고양＋ CUMS 법＋ REM 수면박탈법조모，기중28지완성조모，이차분조위모형조、침자조、약물조、침약조，매조각7지。모형조부조모불간예；침자조취인당、신정、태충（쌍）진행침자；약물조급여사곡림화아보서륜수용액관위；침약조동시결합침자조여약물조적량충간예방식；3조균련속간예7 d。결과 SDD 조모치대서해마조직〔（41.19±5.59）u/ mL〕화혈청〔（34.57±5.53）u/ mL〕적 SOD 활성교정상조〔（60.19±8.85）、（56.20±13.68）u/ mL〕명현하조（P ＜0.05），차치해마조직〔（5.12±1.22）nmol/ mg〕화혈청〔（6.22±0.96）nmol/ mg〕적 MDA 함량교정상조〔（3.49±1.02）、（4.35±1.55）nmol/ mg〕명현상조（P ＜0.05）；침자조해마조직〔（52.29±11.37）u/ mL〕화혈청〔（54.32±6.95）u/ mL〕SOD 활성균교모형조유명현적상조（P ＜0.05），차부침자조적해마조직〔（3.86±0.80）nmol/ mg〕MDA 함량교모형조득도료명현적하조（P ＜0.05）；약물조부상조료혈청 SOD 활성〔（44.70±5.31）u/ mL，P ＜0.05）〕；침약조상조료해마조직〔（51.95±7.58）u/ mL〕화혈청〔（48.23±6.70）u/ mL〕적 SOD 활성（P ＜0.05）。결론 SDD 조모능명현가속쇠로；3충간예수단균유일정적항쇠로작용，기중침자조최우，침약조차지。
Objective Observation on acupuncture on hippocampus and serum SOD Activity and MDA content of SDD Rats. Methods The 8 normal Sprague Dawley rats were caged together normally. The 32 Sprague Dawley rats were underwent a 21 d solitary raised + CUMS + REM sleep deprivation method modeling. The 28 rats successfully comple-ted the modeling,and divided into model group,acupuncture group,medicine group,acupuncture with medicine group, each group with 7 rats. Model group rats,only modeling without intervention. Acupuncture group rats were acupuncture on acupoint Yintang,Shenting and Taichong(both)each day for 20 min with a 15 s stimulation every 10 min. Medi-cine group rats were gavaged administration with Zoloft and Alprazolam solution. Acupuncture with medicine group combined acupuncture and medicine intervention. Three intervention groups were given intervention for 7 days. Results SOD activity of hippocampal tissue(41. 19 ± 5. 59)u / mL and serum(34. 57 ± 5. 53)/ mL of DD rats was signifi-cantly lower than the normal rats(P < 0. 05),and hippocampus tissue and serum MDA content significantly increased than the normal(60. 19 ± 8. 85,56. 20 ± 13. 68)u / mL rats(P < 0. 05);Acupuncture group’s SOD activity of hipp-ocampal tissue(5. 12 ± 1. 22)nmol/ mg and serum(6. 22 ± 0. 96)nmol/ mg were up regulated than the model group (3. 49 ± 1. 02),(4. 35 ± 1. 55)nmol/ mg(P < 0. 05),and only acupuncture group’s MDA content in hippocampus has been significantly down regulated(P < 0. 05);Medicine group’s SOD(52. 29 ± 11. 37)u / ml activity of serum (54. 32 ± 6. 95)u / ml were down regulated(P < 0. 05);Acupuncture combined with medicine group’s SOD activity (44. 70 ± 5. 31)u / mL,P < 0. 05,of hippocampal tissue(51. 95 ± 7. 58)u / ml and serum(48. 23 ± 6. 70)u / ml were up regulated(P < 0. 05). Conclusion Sleep deprivation depression modeling can significantly accelerate ag-ing;3 ways of interventions on SDD rats have some anti-aging effects. And acupuncture group has the best effect with acupuncture combined with medicine group followed.