CAJ | 학술논문

目的：从 IL-4/ STAT6信号通路入手，以该通路负反馈调节因子 SOCS1和关键因子 STAT6为检测指标，多角度探查溃疡性结肠炎的发生与 IL-4的关系。方法将实验动物分为6组，采用免疫法造模，用免疫组化法检测大鼠 SOCS1、STAT6的活性。结果模型组实验大鼠 SOCS1活性（0.4855±0.1780）显著低于正常组（0.6552±0.1874），P ＜0.05；而 STAT6活性（0.8030±0.2082）则明显高于正常组（0.5893±0.1919）， P ＜0.05。四逆散能显著增加实验大鼠 SOCS1活性（0.5765±0.2080）（P ＜0.05），降低 STAT6活性（0.5798±0.1526）（P ＜0.05）；柴芍配伍（0.5625±0.2757）对 SOCS1活性的影响与四逆散（0.5765±0.2080）比较差异无统计学意义，对 STAT6活性（0.5294±0.1443）表达的影响显著优于四逆散（0.5798±0.1526）（P ＜0.05）；芍枳甘配伍（0.6169±0.2006）对 STAT6活性的影响作用明显，与四逆散（0.5798±0.1526）比较差异无统计学意义；柴枳甘配伍对 SOCS1（0.5091±0.2279）、STAT6（0.7561±0.2636）的影响与模型组（0.4855±0.1780；0.8030±0.2082）比较差异无统计学意义。结论四逆散能够通过刺激 SOCS1活性，抑制 STAT6活性表达来调节 IL-4/ STAT6通路，进而干预实验性溃疡性结肠炎。其中，柴芍配伍作用明显；芍枳甘配伍对 STAT6活性的影响作用明显，但对 SOCS1的影响不显著；柴枳甘配伍对 SOCS1、STAT6的影响均不显著。
목적：종 IL-4/ STAT6신호통로입수，이해통로부반궤조절인자 SOCS1화관건인자 STAT6위검측지표，다각도탐사궤양성결장염적발생여 IL-4적관계。방법장실험동물분위6조，채용면역법조모，용면역조화법검측대서 SOCS1、STAT6적활성。결과모형조실험대서 SOCS1활성（0.4855±0.1780）현저저우정상조（0.6552±0.1874），P ＜0.05；이 STAT6활성（0.8030±0.2082）칙명현고우정상조（0.5893±0.1919）， P ＜0.05。사역산능현저증가실험대서 SOCS1활성（0.5765±0.2080）（P ＜0.05），강저 STAT6활성（0.5798±0.1526）（P ＜0.05）；시작배오（0.5625±0.2757）대 SOCS1활성적영향여사역산（0.5765±0.2080）비교차이무통계학의의，대 STAT6활성（0.5294±0.1443）표체적영향현저우우사역산（0.5798±0.1526）（P ＜0.05）；작지감배오（0.6169±0.2006）대 STAT6활성적영향작용명현，여사역산（0.5798±0.1526）비교차이무통계학의의；시지감배오대 SOCS1（0.5091±0.2279）、STAT6（0.7561±0.2636）적영향여모형조（0.4855±0.1780；0.8030±0.2082）비교차이무통계학의의。결론사역산능구통과자격 SOCS1활성，억제 STAT6활성표체래조절 IL-4/ STAT6통로，진이간예실험성궤양성결장염。기중，시작배오작용명현；작지감배오대 STAT6활성적영향작용명현，단대 SOCS1적영향불현저；시지감배오대 SOCS1、STAT6적영향균불현저。
Objective To explore the relation with IL-4 and UC through multi angles,starting with the IL-4 / STAT6 pathway. Methods The experimental animals were divided into 6 groups. We made the model in immune method,de-tected activity of SOCS1 and STAT6 in rats with immune. Results The activity SOCS1 in model group of rats(0. 485 5 ± 0. 178 0)were significantly lower than that of normal group(0. 655 2 ± 0. 187 4)(P < 0. 05),but the activity of STAT6(0. 803 0 ± 0. 208 2)were significantly higher than that in the normal group(0. 589 3 ± 0. 191 9) (P < 0. 05). Sinisan Powders can significantly increase the activity of SOCS1(0. 576 5 ±0. 208 0)in experimental rats (P <0. 05),decrease STAT6 activity(0.579 8 ±0.152 6)(P <0. 05). The effects of Chaishao compatibility(0. 562 5 ± 0.275 7)was no significant difference with SOCS1 and Sinisan(0. 576 5 ±0. 208 0)Powders(P >0. 05),the activity of STAT6(0.529 4 ±0.144 3)was significantly better than the Sinisan(0.579 8 ±0.152 6)Powders(P <0.05). Shaozhigan compatibility effects on activity of STAT6(0. 616 9 ± 0. 200 6)significantly. Chaizhigan compatibility had no significant difference with SOCS1(0.509 1 ±0.227 9),STAT6(0.756 1 ±0.263 6)and the model group(0.485 5 ±0.178 0;0.803 0 ±0.208 2)in effects(P >0.05). Conclusion Sinisan Powders can regulate expression of the IL-4/ STAT6 pathway by stimulating the activity of SOCS1,inhibiting the activity of STAT6,then intervention experiment UC. And the effect of Chaishao compatibility was the best obvious;the effects of Shaozhigan compatibility was obvious on activity of STAT6,but not on SOCS1. The effect of Chaizhigan compatibility were not obvious on SOCS1 and STAT6.