CAJ | 학술논문

从甘肃定西某淀粉加工厂附近土壤中分离得到一株产普鲁兰酶酶源菌 AI-1，通过形态学、生理生化试验及16S rRNA 序列鉴定并对其进行系统发育分析，鉴定为解淀粉芽孢杆菌（Bacillus amyloliquefaciens），对其发酵培养基成分和发酵条件进行了优化。优化后的发酵培养基成分为：可溶性淀粉1.5%，酵母膏1%，蛋白胨1%，NaCl 0.5%，K2 HPO40.1%，MgSO4·7H2 O 0.05%；最佳发酵条件为：培养温度36℃，发酵培养基初始 pH 7.0，接种量8%（V/ V），摇床转速150 r/ min，发酵周期72 h。在此优化条件下，菌株 AI-1发酵所产普鲁兰酶的酶活由最初的2.45 U/ mL提高到了4.52 U/ mL。
종감숙정서모정분가공엄부근토양중분리득도일주산보로란매매원균 AI-1，통과형태학、생리생화시험급16S rRNA 서렬감정병대기진행계통발육분석，감정위해정분아포간균（Bacillus amyloliquefaciens），대기발효배양기성분화발효조건진행료우화。우화후적발효배양기성분위：가용성정분1.5%，효모고1%，단백동1%，NaCl 0.5%，K2 HPO40.1%，MgSO4·7H2 O 0.05%；최가발효조건위：배양온도36℃，발효배양기초시 pH 7.0，접충량8%（V/ V），요상전속150 r/ min，발효주기72 h。재차우화조건하，균주 AI-1발효소산보로란매적매활유최초적2.45 U/ mL제고도료4.52 U/ mL。
A strain AI-1 having Pullulanase activity was isolated from the soil near a starch-processing fac-tory in Dingxi,Gansu province. The optimal culture conditions for enzyme production were determined. Based on 16S rRNA sequence homology analysis and phylogenetic analysis. AI-1 was identified as Bacillus amyloliquefaciens,with its physiological and biochemistry characters. And then its fermentation medium composition and fermentation conditions were optimized,the optimum fermentation medium composition were as follows:soluble starch 1. 5% ,yeast extract 1% ,peptone 1% ,NaCl 0. 5% ,K2 HPO4 0. 1% ,Mg-SO4 ·7H2 O 0. 05% . Under the optimum fermentation condition of initial 36 ℃,pH 7. 0,150 r/ min and an inoculum size of 8%(V/ V)for 72 h,the activity of Pullulanase produced by AI-1 reached the peak at 4. 52 U/ mL from the initial activity of 2. 45 U/ mL.