CAJ | 학술논문

实验研究了在硅球表面用引发－转移－终止剂（iniferter）法制备氯霉素分子印迹聚合物。实验使用硅烷化试剂3－氨基丙基三乙氧基硅烷（APTES）、对氯甲基苯甲酰氯、二乙基二硫代氨基甲酸钠分别在硅球上接枝使硅球表面连接 iniferter 。然后以甲基丙烯酸为功能单体、乙二醇二甲基丙烯酸酯为交联剂，在光引发下自由基反应生成分子印迹聚合物。该印迹聚合物由于聚合均匀印迹位点暴露，因此与传统表面印迹聚合相比，印迹因子有了一定的提高。最后将聚合物作为色谱固定相填料，利用压柱机压入不锈钢柱中，连接高效液相色谱仪检测分离模板和竞争物的混合溶液，印迹情况良好。
실험연구료재규구표면용인발－전이－종지제（iniferter）법제비록매소분자인적취합물。실험사용규완화시제3－안기병기삼을양기규완（APTES）、대록갑기분갑선록、이을기이류대안기갑산납분별재규구상접지사규구표면련접 iniferter 。연후이갑기병희산위공능단체、을이순이갑기병희산지위교련제，재광인발하자유기반응생성분자인적취합물。해인적취합물유우취합균균인적위점폭로，인차여전통표면인적취합상비，인적인자유료일정적제고。최후장취합물작위색보고정상전료，이용압주궤압입불수강주중，련접고효액상색보의검측분리모판화경쟁물적혼합용액，인적정황량호。
The research aims to prepare chloramphenicol molecularly imprinted polymer on the surface of silica by initiator - transfer agent - terminator (iniferter) .Firstly ,the research uses silica alkylation reagent ,3 - APTES , chloromethyl benzoyl chloride and sodium diethyl dithiocarbamatre to make "iniferter" grafted on the surface of silica bead .Then the research uses methacrylic acid as the functional monomer and ethylene glycol dimethacrylate as the cross - linker ,preparing molecularly surface imprinted polymers under UV light .Compared with the traditional imprinting technique ,imprinting factor has a certain amount of increase ,which is because the polymer's average imprinting sites are exposed .Finally ,the obtained imprinted silica beads are pressed into the stainless steel column as chromatography 's fixed packing material through column press . Connected the high performance liquid chromatography ,the mixed solution of templates and competition is finally separated with good imprinting .