CAJ | 학술논문

建立了高效液相色谱法测定高三尖杉酯碱含量的方法。采用超声波辅助提取高三尖杉酯碱，利用 C18色谱柱，以甲醇：醋酸铵（53：47，V/ V）为流动相，用二极管阵列检测器（检测波长290 nm）对高三尖杉酯碱进行测定，流速1.0 mL/ min，柱温25℃。结果表明，在0.12~1.2 mg/ mL 的范围内呈良好的线性关系，相关系数 R =0.99994，回收率为97.02%~99.74%。方法操作简便、快速、灵敏度较高，适用于高三尖杉酯碱的测定。
건립료고효액상색보법측정고삼첨삼지감함량적방법。채용초성파보조제취고삼첨삼지감，이용 C18색보주，이갑순：작산안（53：47，V/ V）위류동상，용이겁관진렬검측기（검측파장290 nm）대고삼첨삼지감진행측정，류속1.0 mL/ min，주온25℃。결과표명，재0.12~1.2 mg/ mL 적범위내정량호적선성관계，상관계수 R =0.99994，회수솔위97.02%~99.74%。방법조작간편、쾌속、령민도교고，괄용우고삼첨삼지감적측정。
A HPLC method is established for determination of Homoharringtonine in Cephalotaxus sinesis. Homoharringtonine was extracted by ultrasonic,separated on column C18 with methanol-ammonium acetate (53 : 47,V/ V)as mobile phase,detected by diode array detector(290 nm). And the flow rate was 1. 0 mL/ min,the column temperature is 25 ℃. The results showed that hydroquinone is considered as having a good linear relation in the range of 0. 12 ~ 1. 2 mg / mL,the respective correlation of Homohar-ringtonine was 0. 999 94,the recovery rate was 97. 02% ~ 99. 74% . It’s an easily carry out,rapid way with high sensitivity to determine the content of Homoharringtonine.