CAJ | 학술논문

目的研究侧脑室注射Apelin-13对脑缺血再灌注损伤大鼠神经缺损程度及脑内(Yes-associated protein,YAP)表达的影响.方法健康成年Wistar雄性大鼠,采用双盲法随机分为对照组、脑缺血-再灌注组和Apelin-13干预组,每组3只大鼠.给予大鼠禁食禁水12 h后,采用线栓法制作大鼠脑缺血-再灌注模型,缺血1h,再灌注24 h,Apelin-13干预组于再灌注后经侧脑室注射Apelin-13.检测大鼠神经功能缺损程度进行行为学评定,取材进行TTC染色检测大鼠脑梗死体积,蛋白免疫印迹法检测大鼠脑组织各部位YAP的表达变化.结果与脑缺血再灌注组相比[(2.67±0.33)分],Apelin-13干预组大鼠神经缺损评分[(1.67±0.33)分]有所降低,症状减轻,差异有统计学意义(P＜0.05),脑梗死体积减小[(30.60± 1.42)％ vs (23.70±2.20)％,P＜0.05],脑组织各部位YAP表达均上调,差异有统计学意义(P＜0.05).结论 Apelin-13通过调节YAP的表达在大鼠脑缺血-再灌注损伤中发挥治疗效果,具有一定的神经保护作用.
목적 연구측뇌실주사Apelin-13대뇌결혈재관주손상대서신경결손정도급뇌내(Yes-associated protein,YAP)표체적영향.방법 건강성년Wistar웅성대서,채용쌍맹법수궤분위대조조、뇌결혈-재관주조화Apelin-13간예조,매조3지대서.급여대서금식금수12 h후,채용선전법제작대서뇌결혈-재관주모형,결혈1h,재관주24 h,Apelin-13간예조우재관주후경측뇌실주사Apelin-13.검측대서신경공능결손정도진행행위학평정,취재진행TTC염색검측대서뇌경사체적,단백면역인적법검측대서뇌조직각부위YAP적표체변화.결과 여뇌결혈재관주조상비[(2.67±0.33)분],Apelin-13간예조대서신경결손평분[(1.67±0.33)분]유소강저,증상감경,차이유통계학의의(P＜0.05),뇌경사체적감소[(30.60± 1.42)％ vs (23.70±2.20)％,P＜0.05],뇌조직각부위YAP표체균상조,차이유통계학의의(P＜0.05).결론 Apelin-13통과조절YAP적표체재대서뇌결혈-재관주손상중발휘치료효과,구유일정적신경보호작용.
Objective To investigate the neuroprotective effect of lateral intracerebroventricular injection of Apelin-13 on the YAP expression and the cerebral ischemia/reperfusion (I/R) injury in Wistar rats.Methods Healthy adult male Wistar rats were randomly divided into the sham group, cerebral I/R group and Apelin-13 treatment group.The middle cerebral artery occlusion model was established with ischemia for 1 hour and reperfusion for 24 hours after restricting food and water intake for 12 hours.Apelin-13 was injected into rats' lateral ventricles of Apelin-13 treatment group after reperfusion.Neural function defects was assessed.The volume of infarction was evaluated by TTC staining.The expression levels of YAP were detected by western blot.Results Compared with the cerebral I/R group,the rats in the Apelin-13 treatment group had abetter neurologic score ((2.67±0.33) vs (1.67±0.33) , P＜0.05), the infarction volume was decreased ((30.60± 1.42) ％ vs (23.70± 2.20) ％,P＜0.05) , and YAP expression level was increased in each part of the cerebral tissue(P＜0.05).Conclusion Apelin-13 has a neuroprotective effect,which plays the therapeutic effect by regulating the expression of YAP on cerebral ischemia/ reperfusion (I/R) injury in Wistar rats.