中国卫生标准管理
中國衛生標準管理
중국위생표준관리
China Health Standard Management
2015年
27期
139-140
,共2页
苗岩%欧春怡%王伟%李卓%王学斌%齐绍伟
苗巖%歐春怡%王偉%李卓%王學斌%齊紹偉
묘암%구춘이%왕위%리탁%왕학빈%제소위
苍白密螺旋体%先天性梅毒%蛋白印迹技术%实时荧光定量PCR
蒼白密螺鏇體%先天性梅毒%蛋白印跡技術%實時熒光定量PCR
창백밀라선체%선천성매독%단백인적기술%실시형광정량PCR
Treponema palidum%Congenital syphilis%Protein imprinting technology%Real-time fluorescent quantitative PCR
目的 研究先天梅毒实验室检测实用性、特异性的方法,应用于新生儿梅毒的早期诊断.方法 应用国内外最新的梅毒检测技术,以梅毒螺旋体明胶颗粒凝集试验(TPPA)与甲苯胺红不加热血清试验 (TRUST)作为基础实验,辅以其它特异性诊断实验,达到快速、准确、特异的诊断目的.结果 2010~2014年全院共检测门诊及住院患者66 338例,其中,产科住院孕产妇30 225例,产科门诊孕产妇10 250例,针对性检测临床疑似新生儿梅毒感染患儿189例,其中住院孕产妇双阳性率(TPPA、TRUST均阳性)0.36%,产科门诊孕产妇双阳性率0.63%,疑似新生儿梅毒感染患儿阳性率19.58%.检出新生儿梅毒患儿11例,约占住院孕产妇0.36‰.结论 使用逆向操作法应用于常规梅毒组合检测,采用蛋白印迹技术针对性检测,提高了孕产妇及新生儿梅毒检测的特异性,应用于新生儿胎传梅毒的早期诊断可为临床诊断先天梅毒提供依据.
目的 研究先天梅毒實驗室檢測實用性、特異性的方法,應用于新生兒梅毒的早期診斷.方法 應用國內外最新的梅毒檢測技術,以梅毒螺鏇體明膠顆粒凝集試驗(TPPA)與甲苯胺紅不加熱血清試驗 (TRUST)作為基礎實驗,輔以其它特異性診斷實驗,達到快速、準確、特異的診斷目的.結果 2010~2014年全院共檢測門診及住院患者66 338例,其中,產科住院孕產婦30 225例,產科門診孕產婦10 250例,針對性檢測臨床疑似新生兒梅毒感染患兒189例,其中住院孕產婦雙暘性率(TPPA、TRUST均暘性)0.36%,產科門診孕產婦雙暘性率0.63%,疑似新生兒梅毒感染患兒暘性率19.58%.檢齣新生兒梅毒患兒11例,約佔住院孕產婦0.36‰.結論 使用逆嚮操作法應用于常規梅毒組閤檢測,採用蛋白印跡技術針對性檢測,提高瞭孕產婦及新生兒梅毒檢測的特異性,應用于新生兒胎傳梅毒的早期診斷可為臨床診斷先天梅毒提供依據.
목적 연구선천매독실험실검측실용성、특이성적방법,응용우신생인매독적조기진단.방법 응용국내외최신적매독검측기술,이매독라선체명효과립응집시험(TPPA)여갑분알홍불가열혈청시험 (TRUST)작위기출실험,보이기타특이성진단실험,체도쾌속、준학、특이적진단목적.결과 2010~2014년전원공검측문진급주원환자66 338례,기중,산과주원잉산부30 225례,산과문진잉산부10 250례,침대성검측림상의사신생인매독감염환인189례,기중주원잉산부쌍양성솔(TPPA、TRUST균양성)0.36%,산과문진잉산부쌍양성솔0.63%,의사신생인매독감염환인양성솔19.58%.검출신생인매독환인11례,약점주원잉산부0.36‰.결론 사용역향조작법응용우상규매독조합검측,채용단백인적기술침대성검측,제고료잉산부급신생인매독검측적특이성,응용우신생인태전매독적조기진단가위림상진단선천매독제공의거.
Objective Practical and specificity of laboratory testing on congenial syphilis are to be researched in order to apply it to diagnose early-stage congenial syphilis in new-born babies.Methods Application of abroad latest laboratory testing technique based on TPPA and TRUST, and supplemented other specific laboratory tests in order to make the rapid,accurate and specified diagnosis.Results There are totaly 66 338 patients treated clinicaly and hospitalized from 2010 to 2014,including 30 223 cases of hospitalized pregnant patients,10 250 cases of clinicaly treated pregnant patients and 189 cases of clinicaly suspected congenial syphilis new-born babies. TPPA and TRUST of hospitalized pregnant patients were up to 0.36%,while,TPPA and TRUST of clinicaly treated pregnant patients were up to 0.63%. And the positive rate of suspected newborn babies infected with congenital syphilis was 19.58%,there are 11 newborn babies diagnosed as congenital syphilis,accounting for 0.36‰ of pregnant patients in total.Conclusion Application of reverse operation to test routine syphilis and application of western blotting to test specific syphilis are conducive to increasing specificity of syphilis test for pregnant women as wel as newborn babies,and what's more,the early-stage diagnosis of newborn babies could provide basis for clinical diagnosis of congenital syphilis.
