CAJ | 학술논문

目的探索姜油树脂对辐射后间充质干细胞中核转录因子E2相关因子2(Nrf2)及其下游靶基因表达变化的影响.方法采用MTT、实时PCR以及Western Blot检测不同浓度姜油树脂、不同时间点处理后Nrf2及其下游靶基因血红素加氧酶(HO1)和还原型辅酶Ⅰ醌类氧化还原酶(NQO1)的表达变化.结果姜油树脂对辐射后间充质干细胞中Nrf2基因本身的影响并不大,但在给予姜油树脂24h后,10-5和10-4g/ml姜油树脂联合照射组下游靶基因HO1和NQO1的mRNA及蛋白水平均有所上升.结论姜油树脂不影响Nrf2的转录和表达,但可提高其下游抗氧化基因的表达,发挥辐射防护作用.
목적 탐색강유수지대복사후간충질간세포중핵전록인자E2상관인자2(Nrf2)급기하유파기인표체변화적영향.방법 채용MTT、실시PCR이급Western Blot검측불동농도강유수지、불동시간점처리후Nrf2급기하유파기인혈홍소가양매(HO1)화환원형보매Ⅰ곤류양화환원매(NQO1)적표체변화.결과 강유수지대복사후간충질간세포중Nrf2기인본신적영향병불대,단재급여강유수지24h후,10-5화10-4g/ml강유수지연합조사조하유파기인HO1화NQO1적mRNA급단백수평균유소상승.결론 강유수지불영향Nrf2적전록화표체,단가제고기하유항양화기인적표체,발휘복사방호작용.
Objective To explore ginger oleoresin induced the change of expression of nuclear factor-erythroid 2 p45-related factor 2(Nrf2) and its target genes in mesenchymal stem cells after radiation damage.Methods A combined approach include MTT, real time-PCR and Western blot were used to test the changes on expression level of Nrf2 and its downstream genes, including heme oxygenase-1 (HO1) and NAD (P) H : quinone oxidoreductase 1 (NQO1).Results The results showed that ginger oleoresin has little effect on Nrf2.However, the expression of HO1 and NQO1 increased in the 10-5, 10-4 g/ml ginger oleoresin group when incubated 24 h after radiation.Conclusions Ginger oleoresin has no effect on the transcription and expression of Nrf2, but can increase the expression of the target antioxidant genes of Nrf2.Ginger oleoresin may be a potential protective agent in the future.