CAJ | 학술논문

目的比较12C重离子与γ射线对人外周血淋巴细胞Peng-EBV增殖抑制率、细胞周期和凋亡率的影响.方法对Peng-EBV细胞分别进行2.0 Gy的12C重离子和γ射线照射,通过MTS法检测细胞增殖变化,流式细胞术检测细胞凋亡率和周期分布.结果 2.0Gy重离子射线和γ射线作用后,Peng-EBV细胞的增殖抑制率均显著升高.与对照组相比,2.0 Gy射线照射后24、48、72 h细胞增殖抑制率差异有统计学意义(重离子射线:t=10.53、4.11、37.30,P＜0.05;γ射线:t=7.18、8.26、26.11,P＜0.05).12C重离子对细胞的增殖抑制率在照射后72 h内呈时间依赖性升高,而γ射线则在照射后48h时增殖抑制率最高,而后降低.重离子射线照射后12h和24h,细胞凋亡率分别较对照组增加了7.92和13.41倍(t=7.09、4.13,P＜0.05);而γ射线照射后12 h和24 h,细胞凋亡率分别为对照组的2.43和5.64倍(t=8.98、20.52,P＜0.05).重离子射线照射后12h和24 h,细胞G2/M期阻滞程度分别较对照组增加5.11和11.77倍(t=12.06、100.08,P＜0.05);而γ射线照射后12 h和24 h,细胞G2/M期阻滞程度分别为对照组的5.82和7.68倍(t=19.35、10.30,P＜0.05).结论 12C重离子对人外周血淋巴细胞造成的生物学效应远高于γ射线.
목적 비교12C중리자여γ사선대인외주혈림파세포Peng-EBV증식억제솔、세포주기화조망솔적영향.방법 대Peng-EBV세포분별진행2.0 Gy적12C중리자화γ사선조사,통과MTS법검측세포증식변화,류식세포술검측세포조망솔화주기분포.결과 2.0Gy중리자사선화γ사선작용후,Peng-EBV세포적증식억제솔균현저승고.여대조조상비,2.0 Gy사선조사후24、48、72 h세포증식억제솔차이유통계학의의(중리자사선:t=10.53、4.11、37.30,P＜0.05;γ사선:t=7.18、8.26、26.11,P＜0.05).12C중리자대세포적증식억제솔재조사후72 h내정시간의뢰성승고,이γ사선칙재조사후48h시증식억제솔최고,이후강저.중리자사선조사후12h화24h,세포조망솔분별교대조조증가료7.92화13.41배(t=7.09、4.13,P＜0.05);이γ사선조사후12 h화24 h,세포조망솔분별위대조조적2.43화5.64배(t=8.98、20.52,P＜0.05).중리자사선조사후12h화24 h,세포G2/M기조체정도분별교대조조증가5.11화11.77배(t=12.06、100.08,P＜0.05);이γ사선조사후12 h화24 h,세포G2/M기조체정도분별위대조조적5.82화7.68배(t=19.35、10.30,P＜0.05).결론 12C중리자대인외주혈림파세포조성적생물학효응원고우γ사선.
Objective To compare the effects of carbon heavy ion and γ ray on cell survival, cell cycle and cell apoptosis.Methods Peng-EBV cells were irradiated by carbon heavy ion and X-ray.MTS was used to detect the cell survival.Cell cycle and apoptosis were analyzed by flow cytometry.Results The proliferation inhibition rate of Peng-EBV cell was significantly increased after 2.0 Gy heavy ion rays and γ rays.Compared with the control group, the cell proliferation inhibition rate difference was statistically significant after 2.0 Gy rays at 24 h, 48 h, 72 h (heavy rays: t=10.53, 4.11, 37.30, all P＜0.05;γ rays: t=7.18, 8.26, 26.11, all P＜0.05).The cell proliferation inhibition rate showed a time-dependent increase, and reached its peak at 72 h after heavy ion rays, while the cell proliferation inhibition rate after γ ray reached its peak at 48 h, and then decreased.Compared with the control group, the apoptosis rate of Peng-EBV cell increased 7.92 and 13.41 times respectively at 12 h and 24 h after 2.0 Gy heavy ion rays (t=7.09, 4.13, both P＜0.05), while it was 2.43 and 5.64 times after 2.0 Gy γ rays (t=8.98, 20.52, P＜ 0.05).Compared with the control group, the G2/M phase arrest increased 5.11 and 11.77 times respectively at 12 h and 24 h after 2.0 Gy heavy ion rays (t=12.06, 100.08, both P＜0.05), however, it was 5.82 and 7.68 times after 2.0 Gy γ rays(t=19.35, 10.30, both P＜0.05).Conclusion 12C heavy ion irradiation shows more stronger biological effects on human peripheral blood lymphocyte than γ ray.