国际放射医学核医学杂志
國際放射醫學覈醫學雜誌
국제방사의학핵의학잡지
International Journal of Radiation Medicine and Nuclear Medicine
2015年
5期
358-362
,共5页
张俊伶%薛晓蕾%李源%樊赛军
張俊伶%薛曉蕾%李源%樊賽軍
장준령%설효뢰%리원%번새군
辐射,电离%活性氧%细胞凋亡%DNA损伤%富氢水%胸腺细胞
輻射,電離%活性氧%細胞凋亡%DNA損傷%富氫水%胸腺細胞
복사,전리%활성양%세포조망%DNA손상%부경수%흉선세포
Radiation,ionizing%Reactive oxygen species%Apoptosis%DNA damage%Hydrogen-rich water%Thymus cells
目的 探讨富氢水对6 Gy照射小鼠胸腺细胞内活性氧水平、细胞凋亡及DNA损伤程度的影响.方法 实验分为对照组、6 Gy照射组、6 Gy照射+富氢水组;对照组和6 Gy照射组小鼠照射前10 min灌胃正常饮用水0.5 ml,6 Gy照射+富氢水组小鼠灌富氢水0.5 ml,照射后连续灌胃,给予小鼠正常饮用水和富氢水7d,于照射后4、7、15d处死小鼠获取胸腺细胞.采用流式细胞术检测胸腺细胞内活性氧水平、凋亡细胞比例及磷酸化组蛋白H2AX(γ-H2AX)平均荧光强度.结果 与对照组的小鼠比较,6 Gy照射组小鼠在照射后4、7、15d胸腺细胞中的活性氧水平升高,早期凋亡细胞[AnnexinV阳性、碘化丙啶(PI)阴性]和晚期凋亡细胞(AnnexinV阳性、PI阳性)的细胞比例明显增加,γ-H2AX平均荧光强度增加.与6 Gy照射组小鼠相比,6Gy照射+富氢水组小鼠的胸腺细胞中活性氧水平下降,早期凋亡和晚期凋亡的细胞比例降低,细胞内γ-H2AX平均荧光强度则是下降的,差异均具有统计学意义.结论 富氢水可以有效减轻电离辐射引起的胸腺细胞损伤,为富氢水作为辐射损伤防护剂提供了实验依据.
目的 探討富氫水對6 Gy照射小鼠胸腺細胞內活性氧水平、細胞凋亡及DNA損傷程度的影響.方法 實驗分為對照組、6 Gy照射組、6 Gy照射+富氫水組;對照組和6 Gy照射組小鼠照射前10 min灌胃正常飲用水0.5 ml,6 Gy照射+富氫水組小鼠灌富氫水0.5 ml,照射後連續灌胃,給予小鼠正常飲用水和富氫水7d,于照射後4、7、15d處死小鼠穫取胸腺細胞.採用流式細胞術檢測胸腺細胞內活性氧水平、凋亡細胞比例及燐痠化組蛋白H2AX(γ-H2AX)平均熒光彊度.結果 與對照組的小鼠比較,6 Gy照射組小鼠在照射後4、7、15d胸腺細胞中的活性氧水平升高,早期凋亡細胞[AnnexinV暘性、碘化丙啶(PI)陰性]和晚期凋亡細胞(AnnexinV暘性、PI暘性)的細胞比例明顯增加,γ-H2AX平均熒光彊度增加.與6 Gy照射組小鼠相比,6Gy照射+富氫水組小鼠的胸腺細胞中活性氧水平下降,早期凋亡和晚期凋亡的細胞比例降低,細胞內γ-H2AX平均熒光彊度則是下降的,差異均具有統計學意義.結論 富氫水可以有效減輕電離輻射引起的胸腺細胞損傷,為富氫水作為輻射損傷防護劑提供瞭實驗依據.
목적 탐토부경수대6 Gy조사소서흉선세포내활성양수평、세포조망급DNA손상정도적영향.방법 실험분위대조조、6 Gy조사조、6 Gy조사+부경수조;대조조화6 Gy조사조소서조사전10 min관위정상음용수0.5 ml,6 Gy조사+부경수조소서관부경수0.5 ml,조사후련속관위,급여소서정상음용수화부경수7d,우조사후4、7、15d처사소서획취흉선세포.채용류식세포술검측흉선세포내활성양수평、조망세포비례급린산화조단백H2AX(γ-H2AX)평균형광강도.결과 여대조조적소서비교,6 Gy조사조소서재조사후4、7、15d흉선세포중적활성양수평승고,조기조망세포[AnnexinV양성、전화병정(PI)음성]화만기조망세포(AnnexinV양성、PI양성)적세포비례명현증가,γ-H2AX평균형광강도증가.여6 Gy조사조소서상비,6Gy조사+부경수조소서적흉선세포중활성양수평하강,조기조망화만기조망적세포비례강저,세포내γ-H2AX평균형광강도칙시하강적,차이균구유통계학의의.결론 부경수가이유효감경전리복사인기적흉선세포손상,위부경수작위복사손상방호제제공료실험의거.
Objective The apoptosis and DNA damage of thymus cells in mice with total body irradiation of 6 Gy were investigated to determine the effect of hydrogen-rich water on reactive oxygen species (ROS).Methods A total of 15 mice were divided into three groups, namely, un-irradiated control group, 6 Gy irradiation group, and 6 Gy +hydrogen-rich water group.Up to 0.5 ml of hydrogen-rich water was administered to the mice in 6 Gy+hydrogen-rich water group 10 min before irradiation, which was provided once a day within 7 days after irradiation, and the other two groups were administered water instead.Thymus cells were collected at 4, 7, and 15 days after irradiation.Levels of ROS, apoptosis, and mean fluorescence intensity (MFI) of phosphorylated histone H2AX (γ-H2AX) were detected by flow cytometry.Results ROS levels, early (Annexin V-positive and propidium iodide (PI)-negative) and late apoptotic (Annexin V-positive and PI-positive) cells, and MFI of γ-H2AX increased in the thymus of mice irradiated with 6 Gy compared with those in the un-irradiated control group.By contrast, the levels of ROS, apoptotic cells, and MFI of γ-H2AX significantly declined in the thymus of mice administered with 6 Gy + hydrogen-rich water compared with those in the irradiated mice.Conclusion Hydrogen-rich water exerted a promising protective effect on radiation-induced thymus injury.Therefore, hydrogen-rich water is a potential radioprotective agent.
