CAJ | 학술논문

目的研究影响小鼠骨辐射诱导电子顺磁共振(EPR)信号检测的因素.方法将小鼠股骨去除软组织和骨髓,干燥,制成股骨段,经60Co γ射线照射,照射剂量率为5 Gy/min,照射剂量为10 Gy和50 Gy.检测不同干燥程度、检测方向、微波功率和叠加次数等条件下骨EPR的信号强度.结果降低骨样品含水量、设置合适的微波功率、增加叠加次数均可以增加样品信号强度,且不同检测方向下同一份样品的信号强度有一定差异.结论适当的样品预处理和检测参数设定可以提高骨EPR检测的灵敏度,样品多方向检测并取平均值可减弱骨样品不均一产生的影响.
목적 연구영향소서골복사유도전자순자공진(EPR)신호검측적인소.방법 장소서고골거제연조직화골수,간조,제성고골단,경60Co γ사선조사,조사제량솔위5 Gy/min,조사제량위10 Gy화50 Gy.검측불동간조정도、검측방향、미파공솔화첩가차수등조건하골EPR적신호강도.결과 강저골양품함수량、설치합괄적미파공솔、증가첩가차수균가이증가양품신호강도,차불동검측방향하동일빈양품적신호강도유일정차이.결론 괄당적양품예처리화검측삼수설정가이제고골EPR검측적령민도,양품다방향검측병취평균치가감약골양품불균일산생적영향.
Objective To study the influencing factors of detection on ionizing radiation-induced electron paramagnetic resonance (EPR) signal in mice bone.Methods Soft tissue and bone marrow samples of mice femur were collected, and femur sections were dried.The femur sections were irradiated at 10 and 50 Gy by 60Co sources with irradiation dose rate of 5 Gy/min.Bone EPR signal intensity was detected under different degrees of drying, detection direction, and microwave power, as well as various scan times.Results The water content of the bone sample was reduced, and microwave power was set to an appropriate value.Results showed that increasing scan times increased the signal intensity.Additionally, different detection directions of the same sample resulted in differences in signal intensity.Conclusion Appropriate sample preparation and detection parameters can improve bone EPR detection sensitivity.Multidirectional detection of samples and averaging of the results may reduce the effects of inhomogeneity on bone samples.