西南师范大学学报(自然科学版)
西南師範大學學報(自然科學版)
서남사범대학학보(자연과학판)
Journal of Southwest China Normal University (Natural Science Edition)
2015年
10期
181-186
,共6页
徐德林%储士润%李树刚%李娟%乔晓颖%钱刚
徐德林%儲士潤%李樹剛%李娟%喬曉穎%錢剛
서덕림%저사윤%리수강%리연%교효영%전강
白及%种苗快繁%组织培养%天然添加物
白及%種苗快繁%組織培養%天然添加物
백급%충묘쾌번%조직배양%천연첨가물
Bletilla striata (Thunb .) Reichb .f .%seedling rapid propagation%tissue culture%natural additive
白及是现代医药工业和化妆品工业的重要原材料,在基本培养基中添加不同比例的马铃薯、西红柿、香蕉和椰汁,检测种子萌发率和组培苗不同器官的表型数据,比较了不同天然添加物对白及组培苗生长势的影响,研究促进白及生长发育的营养参数.发现添加15%马铃薯利于白及叶片和茎的生长;添加20%的西红柿利于叶片生长,但对根的生长具有一定抑制作用;添加2%的香蕉显著促进白及块茎生长;添加1%的椰汁利于白及根的增殖;15%的马铃薯和1%的椰汁能促进白及植株的快速生长.白及种子在M S+2.0 mg/L IBA+30.0 g/L蔗糖+7.0 g/L琼脂+15%马铃薯培养基上进行萌发,30 d后继代到M S+2.0 mg/L IBA+30.0 g/L蔗糖+7.0 g/L琼脂+1%椰汁培养基上培养,3个月后可以发育成茎粗、根多、苗壮的优质种苗.
白及是現代醫藥工業和化妝品工業的重要原材料,在基本培養基中添加不同比例的馬鈴藷、西紅柿、香蕉和椰汁,檢測種子萌髮率和組培苗不同器官的錶型數據,比較瞭不同天然添加物對白及組培苗生長勢的影響,研究促進白及生長髮育的營養參數.髮現添加15%馬鈴藷利于白及葉片和莖的生長;添加20%的西紅柿利于葉片生長,但對根的生長具有一定抑製作用;添加2%的香蕉顯著促進白及塊莖生長;添加1%的椰汁利于白及根的增殖;15%的馬鈴藷和1%的椰汁能促進白及植株的快速生長.白及種子在M S+2.0 mg/L IBA+30.0 g/L蔗糖+7.0 g/L瓊脂+15%馬鈴藷培養基上進行萌髮,30 d後繼代到M S+2.0 mg/L IBA+30.0 g/L蔗糖+7.0 g/L瓊脂+1%椰汁培養基上培養,3箇月後可以髮育成莖粗、根多、苗壯的優質種苗.
백급시현대의약공업화화장품공업적중요원재료,재기본배양기중첨가불동비례적마령서、서홍시、향초화야즙,검측충자맹발솔화조배묘불동기관적표형수거,비교료불동천연첨가물대백급조배묘생장세적영향,연구촉진백급생장발육적영양삼수.발현첨가15%마령서리우백급협편화경적생장;첨가20%적서홍시리우협편생장,단대근적생장구유일정억제작용;첨가2%적향초현저촉진백급괴경생장;첨가1%적야즙리우백급근적증식;15%적마령서화1%적야즙능촉진백급식주적쾌속생장.백급충자재M S+2.0 mg/L IBA+30.0 g/L자당+7.0 g/L경지+15%마령서배양기상진행맹발,30 d후계대도M S+2.0 mg/L IBA+30.0 g/L자당+7.0 g/L경지+1%야즙배양기상배양,3개월후가이발육성경조、근다、묘장적우질충묘.
B .striata is an important raw material for modern industries of pharmaceutical and cosmetics in Chinese traditional medicine .To explore the parameters of produce and development ,diverse natural addi-tives have been applied to observe the seedling potential grow th in B .striata through tissue culture tech-nique .Phenotypic data of grow th have been measured in the present experimental materials ,depending on MS solution and diverse natural additives of tomato ,potato ,banana and coconut juice .Rapid growths of leaf and stem are observed in MS solution with 15% potato .As compared with significant leaf growth ,in-hibition root growth has been detected in 20% natural additive of tomato .Moreover ,MS solution with 2%banana has been applied to promote stem growth ,by control with notable root proliferation in MS solution with 1% coconut juice .Interestingly ,rapid potential growth has been suffered from MS solution with 15%potato and 1% coconut juice in B .striata .After sows the B .striata seed on the medium of MS+2 .0 mg/L IBA+30 g/L sucrose+7 g/L agar+15% potato for germination with 30 days ,the plantlet is subcultured on the medium of MS+2 .0 mg/L IBA+30 g/L sucrose+7 g/L agar+1% coconut juice for 3 months ,the final cultured seedlings could totally favor for the field culturing with stout stem ,sturdy roots and vigorous growing .