CAJ | 학술논문

采用HPLC对细辛挥发油提取物中马兜铃酸A进行检测分析，并对其含量测定方法进行研究．方法：以十八烷基硅烷键合硅胶为色谱分离填充剂，确定细辛挥发油提取物中马兜铃酸A的检测方法，流动相为乙腈甲酸（1.0％，35∶65）；流速为1.0 mL／min；检测波长250 nm ；柱温35℃．结果：该测定方法系统适用性良好，马兜铃酸A在体积浓度为0.505～2.525μg／m L范围内线性关系良好（ r＝0.9994），且回收率较高，经分析比较，该细辛挥发油提取物中检测未含有马兜铃酸A．结论：该测定方法灵敏准确，适用于细辛挥发油提取物中马兜铃酸A的测定分析，细辛挥发油提取物的使用较为安全．
채용HPLC대세신휘발유제취물중마두령산A진행검측분석，병대기함량측정방법진행연구．방법：이십팔완기규완건합규효위색보분리전충제，학정세신휘발유제취물중마두령산A적검측방법，류동상위을정갑산（1.0％，35∶65）；류속위1.0 mL／min；검측파장250 nm ；주온35℃．결과：해측정방법계통괄용성량호，마두령산A재체적농도위0.505～2.525μg／m L범위내선성관계량호（ r＝0.9994），차회수솔교고，경분석비교，해세신휘발유제취물중검측미함유마두령산A．결론：해측정방법령민준학，괄용우세신휘발유제취물중마두령산A적측정분석，세신휘발유제취물적사용교위안전．
Establishing a HPLC method for analyzing the Aristolochie Acid A of the volatile oil from Asa-rum heterotropoides ,this method is used to determine the content of Aristolochie Acid A in Asarum vola-tile oil through study on extracted Asarum volatile oil .An Agilent Hypersil ODS C18 column has been used .The mobile phase was acetonitrile and containing 0 .1 % formic acid in the water (35∶65 ,V/V ) . The flow rate was 1 .0 ml/min .The UV wave length was set at 250 nm and the column temperature at 30℃ .Results show that applicability of the method system is good .The linear relationship between the concentration ranges at 0 .505-2 .525 μg/ml was good .The result shows that there is not contains Aris-tolochie Acid A in the Asarum volatile oil .It is concluded that the method is stable and highly sensitive for determination of Aristolochie Acid A in the Asarum volatile oil ,which promises the safe usage .