CAJ | 학술논문

目的：探讨外源性 p53上调凋亡调制因子（PUMA）对非小细胞肺癌 A549细胞增殖、凋亡的影响。方法将肺癌A549细胞分为 Control 组、空载体组、PUMA 组（转染 pCEP4-（HA）2-PUMA 质粒），DDP 组（10μg· mL－1）和 PUMA＋DDP 组（转染 pCEP4-（HA）2-PUMA 质粒，加10μg·mL－1顺铂）5个组。细胞生存率和凋亡率分别用 MTT 法和流式细胞仪检测，细胞 PUMA、Bax 及 Bcl-2蛋白表达水平采用 Western blot 方法检测。结果与 Control 组比较：PUMA 组、DDP 组及 PUMA＋DDP 组 A549细胞增殖均显著降低（P ＜0．01），且 PUMA＋DDP组降低程度最强（P ＜0．01）；凋亡率均显著升高（P ＜0．01）；Bax 蛋白水平呈显著性升高（P ＜0．01），Bcl-2蛋白呈显著性下降（P ＜0．01）。结论外源性 PUMA 可抑制肺癌 A549细胞增殖，促进凋亡，其机制与增加细胞 Bax 蛋白表达，降低 Bcl-2蛋白表达有关。
목적：탐토외원성 p53상조조망조제인자（PUMA）대비소세포폐암 A549세포증식、조망적영향。방법장폐암A549세포분위 Control 조、공재체조、PUMA 조（전염 pCEP4-（HA）2-PUMA 질립），DDP 조（10μg· mL－1）화 PUMA＋DDP 조（전염 pCEP4-（HA）2-PUMA 질립，가10μg·mL－1순박）5개조。세포생존솔화조망솔분별용 MTT 법화류식세포의검측，세포 PUMA、Bax 급 Bcl-2단백표체수평채용 Western blot 방법검측。결과여 Control 조비교：PUMA 조、DDP 조급 PUMA＋DDP 조 A549세포증식균현저강저（P ＜0．01），차 PUMA＋DDP조강저정도최강（P ＜0．01）；조망솔균현저승고（P ＜0．01）；Bax 단백수평정현저성승고（P ＜0．01），Bcl-2단백정현저성하강（P ＜0．01）。결론외원성 PUMA 가억제폐암 A549세포증식，촉진조망，기궤제여증가세포 Bax 단백표체，강저 Bcl-2단백표체유관。
ABSTRACT:Objective To investigate the effects of exogenous p53 up-regulated modulator of apoptosis(PUMA)on the proliferation and apoptosis of non-small cell lung cancer A549 cells. Methods Non-small cell lung cancer A549 cells were divided into 5 groups:control group,empty vector group,PUMA group(cells were transfected with pCEP4-(HA)2-PUMA plasmids),DDP group(cells were treated with 10 μg·mL-1 cisplatin),and PUMA+DDPgroup(cells were trans-fected with pCEP4-(HA)2-PUMA plasmids and treated with 10 μg·mL-1 cisplatin).Cell growth and apoptosis were determined by MTT assay and flow cytometry,respectively.The expression of PUMA,Bax and Bcl-2 proteins were detected by Western blot.Results Compared with control group,the proliferation of A549 cells was decreased and the apoptosis of A549 cells was increased in PUMA,DDP and PUMA+DDP groups(P <0.01).Maximum decrease in cell proliferation was observed in PUMA+DDP group(P <0.01).In addition,compared with control group,Bax pro-tein expression was up-regulated and Bcl-2 protein expression was down-regulated in PUMA, DDP and PUMA+DDP groups(P <0.01).Conclusion Exogenous PUMA can inhibit prolifera-tion and promote apoptosis through increasing Bax expression and decreasing Bcl-2 expression in non-small cell lung cancer A549 cells.