CAJ | 학술논문

目的：探讨细胞色素氧化酶P4501A1（CYP1A1）基因多态性与急性淋巴细胞白血病（ALL）儿童大剂量甲氨蝶呤（HD－MTX）化疗后毒副作用的关系。方法：用RT－PCR－变性梯度凝胶电泳（DGGE）及DNA测序技术对51例ALL儿童CYP1A1进行多态性筛查，统计患儿HD－MTX毒副作用表现，分析两者相关性。结果：仅筛查到一个多态位点，即A4889G，4889A／G 各基因型与患儿毒副作用发生均无关（P ＞0．05），高危型患儿较标危型患儿更易发生血小板减少（P ＜0．05）。结论：CYP1A1 A4889G多态性与ALL儿童HD－MTX化疗后各毒副反应无关，血小板减少的发生可能与患儿危险度相关。
목적：탐토세포색소양화매P4501A1（CYP1A1）기인다태성여급성림파세포백혈병（ALL）인동대제량갑안접령（HD－MTX）화료후독부작용적관계。방법：용RT－PCR－변성제도응효전영（DGGE）급DNA측서기술대51례ALL인동CYP1A1진행다태성사사，통계환인HD－MTX독부작용표현，분석량자상관성。결과：부사사도일개다태위점，즉A4889G，4889A／G 각기인형여환인독부작용발생균무관（P ＞0．05），고위형환인교표위형환인경역발생혈소판감소（P ＜0．05）。결론：CYP1A1 A4889G다태성여ALL인동HD－MTX화료후각독부반응무관，혈소판감소적발생가능여환인위험도상관。
Objective To investigate the association between CYP1A1 gene polymorphism and toxicities related to high-does methotrexate of childhood acute leukemia. Methods The SNPs were detected by reverse transcriptional (RT)-PCR-denaturing gradient gel elelctrphoresis combined with direct sequencing in 51 children with acute leukemia. Toxicities were collected thereby. Results Only one SNP,A4889G, was screened in CYP1A1. A4889G polymorphism was not associated with all the toxicities (P > 0.05). High-risk ALL children were more likely to increase the risk of thrombocytopenia compared with standard-risk ALL (P< 0.05). Conclusions CYP1A1 A4889G polymorphism may be not association with all toxicities after HD-MTX ,but the thrombocytopenia may be relevant to the risk degree of ALL children.