CAJ | 학술논문

目的:探讨白细胞(WBC)增高对血红蛋白(Hb)测定结果的影响.方法:收集住院患者血液标本90份,其中WBC值在(10~ <20)× 109/L、(20~ <40)× 109/L、≥40 × 109/L的标本各30份,分别通过XE-2100型血液分析仪和氰化高铁血红蛋白(HiCN)比色法检测各标本的Hb值,其中HiCN比色法首先在660 nm处用分光光度计测其浊度,然后高速离心去除WBC,用上清液进行比色.观察2次测定Hb值的变化.结果:WBC值≥40 × 109/L,会对Hb结果产生影响,且WBC值越高,产生的浊度越大,对Hb的影响越明显(P<0.01).结论:高WBC的存在将使Hb测定值假性增高,使用HiCN稀释血液,高速离心去除WBC后再比色测定可有效消除WBC异常增高对Hb的影响.
목적:탐토백세포(WBC)증고대혈홍단백(Hb)측정결과적영향.방법:수집주원환자혈액표본90빈,기중WBC치재(10~ <20)× 109/L、(20~ <40)× 109/L、≥40 × 109/L적표본각30빈,분별통과XE-2100형혈액분석의화청화고철혈홍단백(HiCN)비색법검측각표본적Hb치,기중HiCN비색법수선재660 nm처용분광광도계측기탁도,연후고속리심거제WBC,용상청액진행비색.관찰2차측정Hb치적변화.결과:WBC치≥40 × 109/L,회대Hb결과산생영향,차WBC치월고,산생적탁도월대,대Hb적영향월명현(P<0.01).결론:고WBC적존재장사Hb측정치가성증고,사용HiCN희석혈액,고속리심거제WBC후재비색측정가유효소제WBC이상증고대Hb적영향.
Objective:To investigate the effects of the increasing of white blood cells ( WBC ) on the hemoglobin ( Hb ) level. Methods:The Hb values of 90 blood specimens[WBC value for (10 to <20) × 109/L in 30 cases,(20 to <40) × 109/L in 30 cases and ≥40 × 109/L in 30 cases] were determined by XE-2100 blood analyzer and the reference method of hemoglobinometry(HiCN colorimetric method) . The turbidity of specimen was measured at the 660 nm wave length of spectrophotometer by HiCN colorimetric method,the WBC in specimens were removed by high-speed centrifuge,and then the Hb values in supernatant were determined by the colorimetric method. The Hb values between before and after centrifuge were compared. Results:The effects of the WBC value≥40 × 109/L on Hb was great. With the increase of the WBC value,the turbidity was more higher,and the effect of Hb was the more obvious (P<0. 05). Conclusions:The high WBC can increase the false Hb value. The effects of the abnormal increasing of WBC on Hb value can be eliminated by the HiCN diluting blood,high-speed centrifuge to removing WBC and colorimetric assay.