CAJ | 학술논문

目的:评估乙型肝炎(乙肝)患者血清乙肝病毒DNA(HBV-DNA)载量与血清免疫和生化标志物之间的关系.方法:收集214例乙肝患者血清,分别用荧光定量 PCR 法、化学发光法和速率法检测血清中的 HBV-DNA、乙肝病毒表面抗原(HBsAg)、乙肝病毒e抗原(HBeAg)和丙氨酸氨基转移酶(ALT),分析各标志物之间的关系.结果:214例中,HBV-DNA阳性率为57.48%;HBeAg阳性率为28.04%.HBV-DNA载量与HBsAg和ALT水平均无相关关系(P>0.05),但与HBeAg水平密切相关(P<0.01),HBV-DNA阳性组的HBeAg和ALT水平均明显高于HBV-DNA阴性组(P<0.01).结论:HBV-DNA载量与HBeAg水平呈正相关关系,与HBsAg和ALT水平无相关性,HBsAg水平作为HBV-DNA的替代方法仍需进一步验证.
목적:평고을형간염(을간)환자혈청을간병독DNA(HBV-DNA)재량여혈청면역화생화표지물지간적관계.방법:수집214례을간환자혈청,분별용형광정량 PCR 법、화학발광법화속솔법검측혈청중적 HBV-DNA、을간병독표면항원(HBsAg)、을간병독e항원(HBeAg)화병안산안기전이매(ALT),분석각표지물지간적관계.결과:214례중,HBV-DNA양성솔위57.48%;HBeAg양성솔위28.04%.HBV-DNA재량여HBsAg화ALT수평균무상관관계(P>0.05),단여HBeAg수평밀절상관(P<0.01),HBV-DNA양성조적HBeAg화ALT수평균명현고우HBV-DNA음성조(P<0.01).결론:HBV-DNA재량여HBeAg수평정정상관관계,여HBsAg화ALT수평무상관성,HBsAg수평작위HBV-DNA적체대방법잉수진일보험증.
Objective:To explore the correlation between hepatitis B virus(HBV)-DNA load and routine serum markers in patients with HBV infection. Methods:The serum HBV-DNA,hepatitis B virus surface antigen(HBsAg),hepatitis B virus e antigen(HBeAg) and alanine aminotransferase ( ALT ) in 214 patients with HBV infection were detected using TaqMan real time polymerase chain reaction,chemiluminescence and rate assay. The relationship between the markers were analyzed. Results:Among the 214 patients,the positive rates of HBV-DNA and HBeAg were 57. 48% and 28. 04%,respectively. The HBV-DNA load was not related to HBsAg and ALT(P>0. 05),and was closely related to the HBeAg level(P<0. 01). The levels of HBeAg and ALT in positive HBV-DNA group were significantly higher than those in negative HBV-DNA group(P<0. 01). Conclusions:The HBV-DNA load is positive correlation with the HBeAg level,and is not correlation with HBsAg and ALT. The HBsAg level standing for HBsAg still nends be further validated.