食品研究与开发
食品研究與開髮
식품연구여개발
Food Research and Development
2015年
19期
12-15
,共4页
焦岩%常影%余世锋%孙晓宏%宋春丽
焦巖%常影%餘世鋒%孫曉宏%宋春麗
초암%상영%여세봉%손효굉%송춘려
大果沙棘总黄酮%抗氧化%抑菌活性
大果沙棘總黃酮%抗氧化%抑菌活性
대과사극총황동%항양화%억균활성
seabuckthorn flavonoids%antioxidation%antibacterial
研究大果沙棘总黄酮的体外抗氧化和抑菌活性。采用DPPH和ABTS自由基清除率检测大果沙棘总黄酮体外抗氧化活性;通过测定抑菌圈直径和最低抑菌浓度检测大果沙棘总黄酮的抑菌作用。100μg/mL的大果沙棘总黄酮对DPPH和ABTS自由基的清除率分别为65.1%和92.3%,接近芦丁和儿茶素对照品;抑菌试验结果显示:0.125 mg/mL~1.0 mg/mL的大果沙棘总黄酮对大肠杆菌、枯草芽孢杆菌和金黄色葡萄球菌有显著的抑菌效果。
研究大果沙棘總黃酮的體外抗氧化和抑菌活性。採用DPPH和ABTS自由基清除率檢測大果沙棘總黃酮體外抗氧化活性;通過測定抑菌圈直徑和最低抑菌濃度檢測大果沙棘總黃酮的抑菌作用。100μg/mL的大果沙棘總黃酮對DPPH和ABTS自由基的清除率分彆為65.1%和92.3%,接近蘆丁和兒茶素對照品;抑菌試驗結果顯示:0.125 mg/mL~1.0 mg/mL的大果沙棘總黃酮對大腸桿菌、枯草芽孢桿菌和金黃色葡萄毬菌有顯著的抑菌效果。
연구대과사극총황동적체외항양화화억균활성。채용DPPH화ABTS자유기청제솔검측대과사극총황동체외항양화활성;통과측정억균권직경화최저억균농도검측대과사극총황동적억균작용。100μg/mL적대과사극총황동대DPPH화ABTS자유기적청제솔분별위65.1%화92.3%,접근호정화인다소대조품;억균시험결과현시:0.125 mg/mL~1.0 mg/mL적대과사극총황동대대장간균、고초아포간균화금황색포도구균유현저적억균효과。
The study was purported to research on the antioxidant activities in vitro and antibacterial activities of seabuckthorn flavonoids. The capacities of eliminating 1 ,1-dipheny1-2-picrylhydra-zyl (DPPH·) and the 2,2'-amino-di (2-ethyl-benzothiazoline sulphonic acid-6)ammonium salt (ABTS+) were assayed compared with the standard sample of rutin and catechin controls. The antibacterial activities were studied through the detemination of the antibacterial circle diameter and minimum inhibitory concentration. The seabuckthorn flavonoids in 100μg/mL had a capacities of eliminating DPPH· and ABTS+at the percentages of 65.1%and 92.3 %, respectively, approximate to the rutin and catechin at the same concentration and the antibacterial studies showed strong antibacterial effect against Escherichia coli, Staphylococcus aureus and Bacillus subtilis.