CAJ | 학술논문

目的：探讨粒细胞集落刺激因子(G-CSF)对慢性心肌缺血的保护作用及其机制。方法雄性日本大耳白兔为研究对象，开胸后不全结扎冠状动脉左前降支，建立慢性心肌缺血模型。将成功制备的模型兔随机分为2组，即G-CSF 干预组和对照组。应用流式细胞术测定外周血 CD34+细胞百分率，应用免疫组化法测定心肌组织 CD34+细胞归巢和 vWF 的表达。结果G-CSF 明显提高慢性心肌缺血模型兔的生存率，增加外周血 CD34+细胞百分率，促进缺血心肌组织中 CD34+细胞归巢和 vWF 表达。结论G-CSF 对兔慢性心肌缺血损伤具有明显保护作用，其机制与外周血 CD34+细胞动员和归巢于缺血心肌以及促进血管新生有关。
목적：탐토립세포집락자격인자(G-CSF)대만성심기결혈적보호작용급기궤제。방법웅성일본대이백토위연구대상，개흉후불전결찰관상동맥좌전강지，건립만성심기결혈모형。장성공제비적모형토수궤분위2조，즉G-CSF 간예조화대조조。응용류식세포술측정외주혈 CD34+세포백분솔，응용면역조화법측정심기조직 CD34+세포귀소화 vWF 적표체。결과G-CSF 명현제고만성심기결혈모형토적생존솔，증가외주혈 CD34+세포백분솔，촉진결혈심기조직중 CD34+세포귀소화 vWF 표체。결론G-CSF 대토만성심기결혈손상구유명현보호작용，기궤제여외주혈 CD34+세포동원화귀소우결혈심기이급촉진혈관신생유관。
Objective To investigate the protective effects and mechanisms of granulocyte-colony-stimulating factor (G-CSF)on a rabbit model of chronic myocardial ischemia.Methods Myocardial ischemia models were created by partial ligation of the left anterior descending coronary artery in Japanese white male rabbits.Rabbits were subcutaneously injected with G-CSF (G-CSF group)or saline (control group)for 6 days after myocardial ischemia.The percentage of CD34-positive cells in the peripheral blood was evaluated by flow cytometry,and CD34-positive cells homing and vWF expression in the ischemic myocardium were determined by immunohistochemistry.Results Rabbits in G-CSF group had a higher survival rate than those in control group (P <0.05).Immunohistochemistry of the ischemic myocardium showed that compared with control group,G-CSF group had increased homing of CD34-positive cells on day 7 post-surgery,and more vessels on day 28 post-surgery by anti-von Willebrand factor staining.In addition,we observed an increase in the percentage of CD34-positive cells in the peripheral blood in G-CSF group.Conclusion G-CSF produces an obvious protective effect against chronic myocardial ischemia in rabbits by increasing stem cell mobilization,homing to ischemic myocardium and accelerating neovascularization.