食品研究与开发
食品研究與開髮
식품연구여개발
Food Research and Development
2015年
20期
92-95
,共4页
耿晓玲%蔡莉%王均华%王朋
耿曉玲%蔡莉%王均華%王朋
경효령%채리%왕균화%왕붕
L-鸟氨酸%废菌渣%正交试验%工艺条件
L-鳥氨痠%廢菌渣%正交試驗%工藝條件
L-조안산%폐균사%정교시험%공예조건
L-ornithine%fermentation residues%orthogonal array design%process conditions
以L-鸟氨酸发酵废菌渣水解固形物为有机氮源，进行L-鸟氨酸发酵工艺条件的研究，实验结果发现最佳回用工艺条件为：水解时间20 h，水解温度90.0℃，水解液加入量6.5 g/100 mL，6.0 mol/L硫酸溶液（mL）与含水废菌渣固形物（g）之比1∶2.5。在上述条件下，L-鸟氨酸产量达到了37.47 g/L，与酵母膏为有机氮源进行的鸟氨酸发酵差异不明显，且产量稳定。
以L-鳥氨痠髮酵廢菌渣水解固形物為有機氮源，進行L-鳥氨痠髮酵工藝條件的研究，實驗結果髮現最佳迴用工藝條件為：水解時間20 h，水解溫度90.0℃，水解液加入量6.5 g/100 mL，6.0 mol/L硫痠溶液（mL）與含水廢菌渣固形物（g）之比1∶2.5。在上述條件下，L-鳥氨痠產量達到瞭37.47 g/L，與酵母膏為有機氮源進行的鳥氨痠髮酵差異不明顯，且產量穩定。
이L-조안산발효폐균사수해고형물위유궤담원，진행L-조안산발효공예조건적연구，실험결과발현최가회용공예조건위：수해시간20 h，수해온도90.0℃，수해액가입량6.5 g/100 mL，6.0 mol/L류산용액（mL）여함수폐균사고형물（g）지비1∶2.5。재상술조건하，L-조안산산량체도료37.47 g/L，여효모고위유궤담원진행적조안산발효차이불명현，차산량은정。
Hydrolysis solids of L-ornithine fermentation residues were used as the organic nitrogen source to study the production of L-ornithine by fermentation.The optimized conditions of reuse process were as follows:hydrolysis time 20 h,hydrolysis temperature 90.0℃,hydrolysate 6.5 g/100 mL,the ratio was 1∶2.5 of 6.0 mol/L H2SO4 solution (mL):fungi residues solids containing water (g). Finally,37.47 g/L of L-ornithine was obtained under these conditions,which was no difference compared with the control group.The yield of the L-ornithine obtained from the continuous reuse process was stable.
