北京中医药大学学报
北京中醫藥大學學報
북경중의약대학학보
Journal of Beijing University of Traditional Chinese Medicine
2015年
10期
694-697,721,724
,共6页
王岩飞%李姝玉%张达%高誉珊%张淑静%王媛媛%吴莹%贾德贤%王谦
王巖飛%李姝玉%張達%高譽珊%張淑靜%王媛媛%吳瑩%賈德賢%王謙
왕암비%리주옥%장체%고예산%장숙정%왕원원%오형%가덕현%왕겸
肾间质纤维化%上皮-间质转化%黄芪注射液%α-平滑肌肌动蛋白%转化生长因子β1%钙依赖性跨膜糖蛋白%小鼠
腎間質纖維化%上皮-間質轉化%黃芪註射液%α-平滑肌肌動蛋白%轉化生長因子β1%鈣依賴性跨膜糖蛋白%小鼠
신간질섬유화%상피-간질전화%황기주사액%α-평활기기동단백%전화생장인자β1%개의뢰성과막당단백%소서
renal interstitial fibrosis%epithelial-mesenchymal transition%astragalus mongholicus injection%alpha-smooth muscle actin%E-cad%transforming growth factorβ1%mouse
目的 探讨黄芪注射液治疗肾间质纤维化的作用机制,为临床中药治疗糖尿病肾间质纤维化提供实验依据. 方法 将16只2型糖尿病模型KKA y 小鼠随机分为模型组和黄芪注射液治疗组,另取8只C57 BL/6 J小鼠作为正常组,治疗组小鼠14周龄开始腹腔注射黄芪注射液. 于24周龄处死小鼠,取肾脏分别观察肾组织病理形态学变化,免疫组织化学法检测小鼠肾组织中α-平滑肌肌动蛋白、钙依赖性跨膜糖蛋白和转化生长因子β1的表达. 结果 可见模型组发生明显的纤维化改变,而经黄芪注射液治疗后,小鼠肾间质基质轻度增多,病变较轻微. 治疗组KKAy 小鼠肾组织中α-平滑肌肌动蛋白、转化生长因子β1的表达较模型组减少,钙依赖性跨膜糖蛋白的表达增多.结论 黄芪注射液可在一定程度上减少α-平滑肌肌动蛋白的表达,抑制KKA y 小鼠肾脏组织中转化生长因子β1表达,上调钙依赖性跨膜糖蛋白的表达水平,阻断并逆转肾小管上皮-间质转化的过程,延缓肾小管上皮细胞转分化的发生发展进程.
目的 探討黃芪註射液治療腎間質纖維化的作用機製,為臨床中藥治療糖尿病腎間質纖維化提供實驗依據. 方法 將16隻2型糖尿病模型KKA y 小鼠隨機分為模型組和黃芪註射液治療組,另取8隻C57 BL/6 J小鼠作為正常組,治療組小鼠14週齡開始腹腔註射黃芪註射液. 于24週齡處死小鼠,取腎髒分彆觀察腎組織病理形態學變化,免疫組織化學法檢測小鼠腎組織中α-平滑肌肌動蛋白、鈣依賴性跨膜糖蛋白和轉化生長因子β1的錶達. 結果 可見模型組髮生明顯的纖維化改變,而經黃芪註射液治療後,小鼠腎間質基質輕度增多,病變較輕微. 治療組KKAy 小鼠腎組織中α-平滑肌肌動蛋白、轉化生長因子β1的錶達較模型組減少,鈣依賴性跨膜糖蛋白的錶達增多.結論 黃芪註射液可在一定程度上減少α-平滑肌肌動蛋白的錶達,抑製KKA y 小鼠腎髒組織中轉化生長因子β1錶達,上調鈣依賴性跨膜糖蛋白的錶達水平,阻斷併逆轉腎小管上皮-間質轉化的過程,延緩腎小管上皮細胞轉分化的髮生髮展進程.
목적 탐토황기주사액치료신간질섬유화적작용궤제,위림상중약치료당뇨병신간질섬유화제공실험의거. 방법 장16지2형당뇨병모형KKA y 소서수궤분위모형조화황기주사액치료조,령취8지C57 BL/6 J소서작위정상조,치료조소서14주령개시복강주사황기주사액. 우24주령처사소서,취신장분별관찰신조직병리형태학변화,면역조직화학법검측소서신조직중α-평활기기동단백、개의뢰성과막당단백화전화생장인자β1적표체. 결과 가견모형조발생명현적섬유화개변,이경황기주사액치료후,소서신간질기질경도증다,병변교경미. 치료조KKAy 소서신조직중α-평활기기동단백、전화생장인자β1적표체교모형조감소,개의뢰성과막당단백적표체증다.결론 황기주사액가재일정정도상감소α-평활기기동단백적표체,억제KKA y 소서신장조직중전화생장인자β1표체,상조개의뢰성과막당단백적표체수평,조단병역전신소관상피-간질전화적과정,연완신소관상피세포전분화적발생발전진정.
Objective To observe the effect of Astragalus mongholicus injection on the expression of epithelial-mesenchymal transition-related proteins in KKAy mice with renal interstitial fibrosis. Methods Sixteen KKAy mice were randomly divided into model group ( n =8 ) and Astragalus mongholicus injection treatment group (n=8),and 8 C57BL/6J mice were used as normal control.The mice in treatment group were intraperitoneally given Astragalus mongholicus injection from the 14 th week. The fasting glucose levels were observed at the 16th, 20th, 24th week.The mice were sacrificed at the 24th week.The renal pathological changes were observed under light microscope.The expression of alpha-smooth muscle actin,E-cad and transforming growth factor β1 in the renal tissues were examined by immunohistochemical staining.Results Fibrosis was found in the KKAy mice of model group,while the mice in treatment group showed a slight increase in renal interstitium.Treatment with Astragalus mongholicus injection decreased the protein expression levels of alpha-smooth muscle actin,transforming growth factor β1 in the kidney tissues as compared with those in model group.Conclusion Astragalus mongholicu injection reduces the expression of alpha-smooth muscle actin and restrains the protein expres-sion of transforming growth factorβ1 in the kidney tissue of KKAy mice.These changes may inhibit and reverse the process of epithelial-mesenchymal transition,thus delaying the occurrence and development of renal interstitial fibrosis.
