CAJ | 학술논문

目的观察防风和乌梅配伍前后对肥大细胞脱颗粒以及蛋白酶激活受体2(PAR-2)表达的影响,为药对防风-乌梅抗过敏的研究提供实验依据. 方法体外采用胰蛋白酶刺激小鼠肥大细胞株P815方法建立肥大细胞脱颗粒模型,运用RT-PCR、Western blotting、ELISA等技术,观察防风-乌梅配伍前后对肥大细胞分泌组胺、PAR-2 mRNA和蛋白表达的影响. 结果防风-乌梅配伍后在体外抑制肥大细胞分泌组胺、PAR-2表达的作用明显增强,与细胞模型组比较有统计学差异(P<0.05或P<0.01). 结论防风-乌梅配伍后抑制脱颗粒作用明显增强,下调肥大细胞PAR-2表达可能是药对防风-乌梅抗过敏的配伍机制之一.
목적 관찰방풍화오매배오전후대비대세포탈과립이급단백매격활수체2(PAR-2)표체적영향,위약대방풍-오매항과민적연구제공실험의거. 방법 체외채용이단백매자격소서비대세포주P815방법건립비대세포탈과립모형,운용RT-PCR、Western blotting、ELISA등기술,관찰방풍-오매배오전후대비대세포분비조알、PAR-2 mRNA화단백표체적영향. 결과 방풍-오매배오후재체외억제비대세포분비조알、PAR-2표체적작용명현증강,여세포모형조비교유통계학차이(P<0.05혹P<0.01). 결론 방풍-오매배오후억제탈과립작용명현증강,하조비대세포PAR-2표체가능시약대방풍-오매항과민적배오궤제지일.
Objective By observing the effects fangfeng-wumei pair on degranulation of mast cells and PAR-2 expression before and after the pairing to provide experimental basis for the research on its anti-allergy mechanism.Methods A mast cell degranulation mouse model was established by stimulating mouse mast cell line P815 with trysin in vitro, and the levels of histamine as well as PAR-2mRNA and protein expression of P815 cells affected by fangfeng-wumei pair were measured using RT-PCR, Western blotting and ELISA.Results Fangfeng-wumei pair in vitro significantly inhibited the secretion of histamine and the expression of PAR-2 by mast cells in comparison to cell model group ( P <0.05 or P<0.01 ) .Conclusion The results demonstrated that inhibition of degranulation of mast cells was significantly enhanced by fangfeng-wumei pair, and that the expression of PAR-2 by mast cells might be involved in the anti-allergy mechanism of fangfeng-wumei pair.