食品研究与开发
食品研究與開髮
식품연구여개발
Food Research and Development
2015年
20期
36-39,40
,共5页
郗艳丽%张啟娟%周婧%都耀%王舒然
郗豔麗%張啟娟%週婧%都耀%王舒然
치염려%장계연%주청%도요%왕서연
女贞子%齐墩果酸%高氯酸-香草醛比色法%高效液相色谱法
女貞子%齊墩果痠%高氯痠-香草醛比色法%高效液相色譜法
녀정자%제돈과산%고록산-향초철비색법%고효액상색보법
ligustrum lucidum ait%oleanolic acid%vanillin-perchlorate colorimetry%HPLC method
以女贞子中齐墩果酸的含量为评价指标，研究提取温度、料液比、回流次数对女贞子中齐墩果酸提取含量的影响，香草醛-高氯酸比色法测定其含量。在提取温度85℃，料液比1∶30（g/mL），回流5次的条件下，齐墩果酸的提取含量最高，可达到122.66μg/mL，此时齐墩果酸提取率为4.066%。高效液相法验证提取物为齐墩果酸，此时齐墩果酸DPPH自由基清除率为70.96%。
以女貞子中齊墩果痠的含量為評價指標，研究提取溫度、料液比、迴流次數對女貞子中齊墩果痠提取含量的影響，香草醛-高氯痠比色法測定其含量。在提取溫度85℃，料液比1∶30（g/mL），迴流5次的條件下，齊墩果痠的提取含量最高，可達到122.66μg/mL，此時齊墩果痠提取率為4.066%。高效液相法驗證提取物為齊墩果痠，此時齊墩果痠DPPH自由基清除率為70.96%。
이녀정자중제돈과산적함량위평개지표，연구제취온도、료액비、회류차수대녀정자중제돈과산제취함량적영향，향초철-고록산비색법측정기함량。재제취온도85℃，료액비1∶30（g/mL），회류5차적조건하，제돈과산적제취함량최고，가체도122.66μg/mL，차시제돈과산제취솔위4.066%。고효액상법험증제취물위제돈과산，차시제돈과산DPPH자유기청제솔위70.96%。
With the content of oleanolic acid from ligustrum lucidum ait as evaluating indicator , the effect of extraction temperature, solid-liquid ratio and reflux times on the extraction content was considered. The contents of oleanolic acid were measured by vanillin-perchlorate colorimetry. The optimal extraction processes were as follows:extraction temperature of 85℃, solid-liquid ratio of 1∶30 (g/mL) and reflux times of five. In these conditions, the extraction content of oleanolic acid was 122.66μg/mL, and the extraction ratio was about 4.066 %. Then the extraction was identified as oleanolic acid by HPLC method , and the scavenging rate of DPPH was 70.96%.
