北京中医药大学学报
北京中醫藥大學學報
북경중의약대학학보
Journal of Beijing University of Traditional Chinese Medicine
2015年
10期
709-715
,共7页
周小军%张丽娟%陈江华%汪芸
週小軍%張麗娟%陳江華%汪蕓
주소군%장려연%진강화%왕예
鼻咽癌%气虚%角蛋白%集落刺激因子1受体%细胞周期蛋白依赖激酶
鼻嚥癌%氣虛%角蛋白%集落刺激因子1受體%細胞週期蛋白依賴激酶
비인암%기허%각단백%집락자격인자1수체%세포주기단백의뢰격매
nasopharyngeal carcinoma%qi deficiency%keratin%colony stimulating factor 1 receptor%cyclin dependent kinase
目的 利用蛋白芯片技术研究气虚质及鼻咽癌气虚癌变差异蛋白的表达. 方法 选择具有典型气虚质的鼻咽癌及鼻咽炎各4例作为研究对象,采用可同时检测722个蛋白的Springbio蛋白芯片技术及协方差分析方法,检测气虚质差异蛋白、鼻咽癌差异蛋白以及鼻咽癌气虚癌变差异蛋白的表达情况,探索其特征蛋白. 结果 相比于正常质,气虚质蛋白主要以下调表达为主,下调蛋白19个( P<0.05) ,气虚质下调的蛋白主要包括角蛋白及细胞周期蛋白依赖激酶;相比于鼻咽炎,鼻咽癌蛋白既可上调表达亦可下调表达,上调表达有13个蛋白,下调表达有9个蛋白;CD115/CSF-1R和角蛋白既在气虚质下调,又在鼻咽癌下调,Cdk1/p34cdc2和p170 /MDR-1蛋白在气虚质下调,在鼻咽癌却上调. 结论 气虚质存在着特定的蛋白表达特征,表现为角蛋白及细胞周期蛋白依赖激酶的低表达,集落刺激因子1受体及角蛋白低表达为鼻咽癌气虚癌变特征蛋白.
目的 利用蛋白芯片技術研究氣虛質及鼻嚥癌氣虛癌變差異蛋白的錶達. 方法 選擇具有典型氣虛質的鼻嚥癌及鼻嚥炎各4例作為研究對象,採用可同時檢測722箇蛋白的Springbio蛋白芯片技術及協方差分析方法,檢測氣虛質差異蛋白、鼻嚥癌差異蛋白以及鼻嚥癌氣虛癌變差異蛋白的錶達情況,探索其特徵蛋白. 結果 相比于正常質,氣虛質蛋白主要以下調錶達為主,下調蛋白19箇( P<0.05) ,氣虛質下調的蛋白主要包括角蛋白及細胞週期蛋白依賴激酶;相比于鼻嚥炎,鼻嚥癌蛋白既可上調錶達亦可下調錶達,上調錶達有13箇蛋白,下調錶達有9箇蛋白;CD115/CSF-1R和角蛋白既在氣虛質下調,又在鼻嚥癌下調,Cdk1/p34cdc2和p170 /MDR-1蛋白在氣虛質下調,在鼻嚥癌卻上調. 結論 氣虛質存在著特定的蛋白錶達特徵,錶現為角蛋白及細胞週期蛋白依賴激酶的低錶達,集落刺激因子1受體及角蛋白低錶達為鼻嚥癌氣虛癌變特徵蛋白.
목적 이용단백심편기술연구기허질급비인암기허암변차이단백적표체. 방법 선택구유전형기허질적비인암급비인염각4례작위연구대상,채용가동시검측722개단백적Springbio단백심편기술급협방차분석방법,검측기허질차이단백、비인암차이단백이급비인암기허암변차이단백적표체정황,탐색기특정단백. 결과 상비우정상질,기허질단백주요이하조표체위주,하조단백19개( P<0.05) ,기허질하조적단백주요포괄각단백급세포주기단백의뢰격매;상비우비인염,비인암단백기가상조표체역가하조표체,상조표체유13개단백,하조표체유9개단백;CD115/CSF-1R화각단백기재기허질하조,우재비인암하조,Cdk1/p34cdc2화p170 /MDR-1단백재기허질하조,재비인암각상조. 결론 기허질존재착특정적단백표체특정,표현위각단백급세포주기단백의뢰격매적저표체,집락자격인자1수체급각단백저표체위비인암기허암변특정단백.
Objective To investigate protein expression characteristics of qi-deficiency constitution and qi deficiency-related nasopharyngeal cancer ( NPC) by using protein chip technology.Methods Four ca-ses with nasopharyngeal carcinoma and 4 cases with nasopharyngitis, all of the typical qi-deficiency con-stitution were examined.Springbio protein chip technology with the capacity of detecting 722 proteins was used to detect protein expressions in qi deficiency constitution, sopharyngeal carcinoma and sopharyngeal carcinoma of qi-deficiency type, so as to explore the characteristics of the protein.Results As compared with the healthy subjects, most protein expressions were down-regulated in qi deficiency patients.There had been 19 down-regulated proteins ( p <0 .05 ) , mainly including the keratin protein and cyclin dependent kinase.As compared with nasopharyngitis, 13 proteins were up-regulated while 9 were down-regulated in nasopharyngeal carcinoma protein expressions.CD115/c-fms/CSF-1R/M-CSFR and keratin showed lower expression in nasopharyngeal carcinoma and qi deficiency type.However, Cdk1 /p34cdc2 and p170 /MDR 1 protein were down-regulated in qi deficiency type, but up-regulated in nasopharyngeal carcinoma.Conclusion There is a specific protein expression characteristic in the deficiency constitu-tion, manifesting as lowered expression of keratin and cyclin dependent kinase.Colony stimulating factor 1 receptor and keratin protein low expression are characteristics of qi deficiency related to nasopharyngeal cancer.