食品研究与开发
食品研究與開髮
식품연구여개발
Food Research and Development
2015年
20期
131-133
,共3页
高效液相色谱%槲皮素%山萘酚%白刺籽
高效液相色譜%槲皮素%山萘酚%白刺籽
고효액상색보%곡피소%산내분%백자자
HPLC%quercetin%kaemferol%Nitraria tangutorum Bobr
采用高效液相色谱法测定白刺籽中槲皮素和山萘酚的含量。采用的条件为Hypersil C18（4.6 mm×250 mm，5μm）色谱柱；流动相甲醇-水（含0.4%的磷酸）48∶52的比例线性洗脱，流速1 mL/min；检测波长360 nm；柱温35℃。两种成分均达到基线分离，槲皮素的线性范围为0.02928μg～0.20496μg，山萘酚的线性范围为0.0034μg～0.0119μg；槲皮素的平均回收率为100.7%（RSD=2.1%），山萘酚的平均回收率为98.5%（RSD=1.7%）。
採用高效液相色譜法測定白刺籽中槲皮素和山萘酚的含量。採用的條件為Hypersil C18（4.6 mm×250 mm，5μm）色譜柱；流動相甲醇-水（含0.4%的燐痠）48∶52的比例線性洗脫，流速1 mL/min；檢測波長360 nm；柱溫35℃。兩種成分均達到基線分離，槲皮素的線性範圍為0.02928μg～0.20496μg，山萘酚的線性範圍為0.0034μg～0.0119μg；槲皮素的平均迴收率為100.7%（RSD=2.1%），山萘酚的平均迴收率為98.5%（RSD=1.7%）。
채용고효액상색보법측정백자자중곡피소화산내분적함량。채용적조건위Hypersil C18（4.6 mm×250 mm，5μm）색보주；류동상갑순-수（함0.4%적린산）48∶52적비례선성세탈，류속1 mL/min；검측파장360 nm；주온35℃。량충성분균체도기선분리，곡피소적선성범위위0.02928μg～0.20496μg，산내분적선성범위위0.0034μg～0.0119μg；곡피소적평균회수솔위100.7%（RSD=2.1%），산내분적평균회수솔위98.5%（RSD=1.7%）。
To determination the content of quercetin and rantiliroside of Nitraria tangutorum Bobr by HPLC.The samples were separated with the column of Hypersil C18 (4.6 mm ×250 mm, 5 μm) which was eluted with methanol and water (0.04%phosphoric acid) in 48∶52 with detected wavelength at 360 nm, flow rate at 1 mL/min,column temperature at 35℃. The results indicated that two compounds were basically insolated.The linear ranges of quercetin and kaemferol were 0.029 28 μg-0.204 96 μg,0.003 4 μg-0.011 9 μg respectively;the average recoveries were 100.7%(RSD=2.1%),98.5%(RSD=1.7%) respectively.
