食品研究与开发
食品研究與開髮
식품연구여개발
Food Research and Development
2015年
20期
107-110
,共4页
曲莉%李潇涵%王雪松%高丽君%艾金霞%李明成
麯莉%李瀟涵%王雪鬆%高麗君%艾金霞%李明成
곡리%리소함%왕설송%고려군%애금하%리명성
改良盐析法%柱层析法%线粒体DNA%PCR%鸭肉
改良鹽析法%柱層析法%線粒體DNA%PCR%鴨肉
개량염석법%주층석법%선립체DNA%PCR%압육
improved salt fractionation%column chromatograph%mitochondrial DNA%PCR%duck
探讨肉类线粒体DNA（mitochondrial DNA，mtDNA）提取方法，对7种生肉中的鸭肉成分进行鉴别。应用改良盐析法和柱层析法提取肉类mtDNA，紫外分光光度计检测mtDNA的浓度和纯度，设计鸭肉cyt b特异性引物，采用PCR对7种生肉中的鸭肉成分进行鉴别。结果：两种提取肉类mtDNA的方法均能保证DNA的浓度及纯度达到PCR的要求，柱层析法成本略高于改良盐析法；鸭引物可以从7种生肉中鉴别出鸭源性成分，经过多次重复试验证明了鸭肉引物的特异性，为肉制品质量监控的应用提供了实验基础。
探討肉類線粒體DNA（mitochondrial DNA，mtDNA）提取方法，對7種生肉中的鴨肉成分進行鑒彆。應用改良鹽析法和柱層析法提取肉類mtDNA，紫外分光光度計檢測mtDNA的濃度和純度，設計鴨肉cyt b特異性引物，採用PCR對7種生肉中的鴨肉成分進行鑒彆。結果：兩種提取肉類mtDNA的方法均能保證DNA的濃度及純度達到PCR的要求，柱層析法成本略高于改良鹽析法；鴨引物可以從7種生肉中鑒彆齣鴨源性成分，經過多次重複試驗證明瞭鴨肉引物的特異性，為肉製品質量鑑控的應用提供瞭實驗基礎。
탐토육류선립체DNA（mitochondrial DNA，mtDNA）제취방법，대7충생육중적압육성분진행감별。응용개량염석법화주층석법제취육류mtDNA，자외분광광도계검측mtDNA적농도화순도，설계압육cyt b특이성인물，채용PCR대7충생육중적압육성분진행감별。결과：량충제취육류mtDNA적방법균능보증DNA적농도급순도체도PCR적요구，주층석법성본략고우개량염석법；압인물가이종7충생육중감별출압원성성분，경과다차중복시험증명료압육인물적특이성，위육제품질량감공적응용제공료실험기출。
Two methods were used to extract the mtDNA in meat , the best method was determined through comparing the concentration and purity of mtDNA .The specific primers of duck cyt b was designed to identify the most common adulterated meat on the market , duck compositions were identified in 7 kinds of raw meat by PCR technology. The results show that the two methods of extracting meat mtDNA can achieve the requirement of the PCR in the concentration and purity of DNA. The cost of column chromatography was higher than the improved salting out method. Duck source composition can be identified from 7 kinds of raw meat by duck primer, the specificity of the duck primers was proved through the repeating experiment. This test provides the experimental basis for the application for the meat products quality monitoring.
