CAJ | 학술논문

从12株红酵母菌中筛选得到一株生物量和类胡萝卜素产量较高的菌株KC 8，以KC 8为出发菌株，依次经ARTP诱变、亚硝酸钠诱变、紫外诱变、紫外-亚硝酸钠复合诱变，再进行一轮ARTP诱变。测得KC 8类胡萝卜素产量为8.67 mg/L，26 S rDNA测序表明，该菌株与胶红酵母（Rhodotorula mucilaginosa）100%相似性；最终从1000株诱变存活菌株中筛选得到高产类胡萝卜素突变菌株K 4，产量为15.14 mg/L，为出发菌株的1.75倍，经高效液相色谱（HPLC）分析表明该菌株所产类胡萝卜素的主要成分为β-胡萝卜素。
종12주홍효모균중사선득도일주생물량화류호라복소산량교고적균주KC 8，이KC 8위출발균주，의차경ARTP유변、아초산납유변、자외유변、자외-아초산납복합유변，재진행일륜ARTP유변。측득KC 8류호라복소산량위8.67 mg/L，26 S rDNA측서표명，해균주여효홍효모（Rhodotorula mucilaginosa）100%상사성；최종종1000주유변존활균주중사선득도고산류호라복소돌변균주K 4，산량위15.14 mg/L，위출발균주적1.75배，경고효액상색보（HPLC）분석표명해균주소산류호라복소적주요성분위β-호라복소。
One yeast strain (KC 8) from 12 was selected and identified due to relatively high biomass and high production of carotenoids (8.67 mg/L). Sequence of 26 S rDNA indicated that KC 8 had 100%identity with Rhodotorula mucilaginosa. Using KC 8 as a starting strain, a series of mutation methods, ARTP, NaNO2, UV light, combination of NaNO2 and UV light were applied to increase the carotenoids production. Finally, K 4 was obtained from 1 000 strains of screening. The yield of carotenoids by K 4 was 15.14 mg/L , which was 1.75 times of the starting strain, KC 8. High performance liquid chromatography (HPLC) analysis indicated that the main component of carotenoids wasβ-carotene.